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A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis.
Fan, Shu-Jin; Tan, Hong-Kun; Xu, Yu-Cheng; Chen, Yuan-Zhi; Xie, Tian-Ao; Pan, Zhi-Yong; Ouyang, Shi; Li, Qin; Li, Xiao-Yan; Li, Zhen-Xing; Guo, Xu-Guang.
Afiliación
  • Fan SJ; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Tan HK; Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
  • Xu YC; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Chen YZ; Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
  • Xie TA; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Pan ZY; Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
  • Ouyang S; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Li Q; Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
  • Li XY; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Li ZX; Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
  • Guo XG; Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
BMC Infect Dis ; 20(1): 525, 2020 Jul 20.
Article en En | MEDLINE | ID: mdl-32689953
BACKGROUND: Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so an inexpensive, easy to use, and accurate technology is needed. We performed a pooled-analysis to assess the potential of the recently developed loop-mediated isothermal amplification (LAMP) assay for detection of meningococcus. METHODS: Pubmed, Embase, and Web of Science were searched to identify original studies that used the LAMP assay to detect meningococcus. After pooling of data, the sensitivity and specificity were calculated, a summary receiver operating characteristic (SROC) curve was determined, and the area under the SROC curve was computed to determine diagnostic accuracy. Publication bias was assessed using Deek's funnel plot. RESULTS: We examined 14 studies within 6 publications. The LAMP assay had high sensitivity (94%) and specificity (100%) in the detection of meningococcus in all studies. The area under the SROC curve (0.980) indicated high overall accuracy of the LAMP assay. There was no evidence of publication bias. DISCUSSION: The LAMP assay has accuracy comparable to bacterial culture and PCR for detection of meningococcus, but is less expensive and easier to use. We suggest the adoption of the LAMP assay to detect meningococcus, especially in economically depressed areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular / Meningitis Meningocócica / Neisseria meningitidis Tipo de estudio: Diagnostic_studies / Prognostic_studies / Systematic_reviews Límite: Humans Idioma: En Revista: BMC Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular / Meningitis Meningocócica / Neisseria meningitidis Tipo de estudio: Diagnostic_studies / Prognostic_studies / Systematic_reviews Límite: Humans Idioma: En Revista: BMC Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido