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Histone methylation and demethylation are implicated in the transient and sustained activation of the interleukin-1ß gene in murine macrophages.
Imuta, Hiroyuki; Fujita, Daishi; Oba, Shigeyoshi; Kiyosue, Arihiro; Nishimatsu, Hiroaki; Yudo, Kazuo; Suzuki, Etsu.
Afiliación
  • Imuta H; Division of Cardiovascular Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
  • Fujita D; Division of Cardiovascular Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
  • Oba S; Division of Clinical Epigenetics, Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
  • Kiyosue A; Division of Cardiovascular Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
  • Nishimatsu H; Department of Urology, Fraternity Memorial Hospital, 2-1-11 Yokozuna, Sumida-ku, Tokyo, 130-8587, Japan.
  • Yudo K; Institute of Medical Science, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, 216-8512, Japan.
  • Suzuki E; Institute of Medical Science, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, 216-8512, Japan. esuzuki-tky@umin.ac.jp.
Heart Vessels ; 35(12): 1746-1754, 2020 Dec.
Article en En | MEDLINE | ID: mdl-32676696
Macrophages play a crucial role in the development of atherosclerosis. To explore the mechanism by which macrophages attain a proinflammatory phenotype for a sustained period, we stimulated macrophages with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) and measured the interleukin-1ß (IL-1ß) expression. The IL-1ß expression increased transiently, and its expression lasted for, at least, 1 week after the cessation of LPS and IFN-γ stimulation. At the promoter region of the IL-1ß gene, the demethylation of histone H3 lysine 27 (H3K27) was significantly induced for 1 week after transient stimulation with LPS and IFN-γ. The expression of H3K27 demethylases ubiquitously transcribed tetratricopeptide repeat, X chromosome (UTX) and jumonji domain-containing 3 (JMJD3) increased significantly for 1 week after transient stimulation with LPS and IFN-γ. When the UTX expression was inhibited by using small interfering RNA (siRNA) for UTX, the IL-1ß expression was significantly suppressed in both transient and sustained phases, whereas siRNA for JMJD3 significantly inhibited only the sustained phase of the IL-1ß expression. These results suggested that H3K27 demethylation was implicated in the transient and sustained increase in the IL-1ß expression after LPS and IFN-γ stimulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Activación Transcripcional / Procesamiento Proteico-Postraduccional / Interleucina-1beta / Activación de Macrófagos / Macrófagos Límite: Animals Idioma: En Revista: Heart Vessels Asunto de la revista: CARDIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Activación Transcripcional / Procesamiento Proteico-Postraduccional / Interleucina-1beta / Activación de Macrófagos / Macrófagos Límite: Animals Idioma: En Revista: Heart Vessels Asunto de la revista: CARDIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón