Predicting carcinogenicity of petroleum distillation fractions using a modified Salmonella mutagenicity assay.
Cell Biol Toxicol
; 2(1): 63-84, 1986 Mar.
Article
en En
| MEDLINE
| ID: mdl-3267446
The Ames Salmonella/microsomal activation mutagenesis assay has been modified to improve sensitivity and reproducibility to complex mixtures derived from the refining and processing of petroleum. Oil samples were dissolved in cyclohexane and subsequently extracted with dimethyl sulfoxide to produce aqueous compatible solutions which readily interact with tester bacteria. Also, the liver homogenate (S-9) and NADP cofactor concentrations were increased and hamster rather than rat liver S-9 was used. The initial slope of the dose response curve relating mutagenicity (revertants per plate) to the dose of extract added was used as an index of mutagenic activity; this slope was obtained through a computerized curve fitting procedure. The modified assay was used to rank 18 oil samples for mutagenic activity; this ranking correlates highly (r = 0.92) with potency rankings of the same samples previously determined from dermal carcinogenicity bioassays. Sensitivity and reproducibility of the assay are sufficient to permit routine use for detecting potential carcinogenic activity of individual refinery streams and blends which contain components boiling above 500 degrees F.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmonella
/
Petróleo
/
Carcinógenos
/
Pruebas de Mutagenicidad
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Cell Biol Toxicol
Asunto de la revista:
TOXICOLOGIA
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Suiza