Your browser doesn't support javascript.
loading
Specific and Non-Invasive Fluorescent Labelling of Extracellular Vesicles for Evaluation of Intracellular Processing by Intestinal Epithelial Cells.
Hansen, Maria S; Gadegaard, Ida S E; Arnspang, Eva C; Blans, Kristine; Nejsum, Lene N; Rasmussen, Jan T.
Afiliación
  • Hansen MS; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
  • Gadegaard ISE; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
  • Arnspang EC; Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.
  • Blans K; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
  • Nejsum LN; Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.
  • Rasmussen JT; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
Biomedicines ; 8(7)2020 Jul 14.
Article en En | MEDLINE | ID: mdl-32674302
The presence of extracellular vesicles (EVs) in milk has gained interest due to their capacity to modulate the infant's intestinal and immune system. Studies suggest that milk EVs are enriched in immune-modulating proteins and miRNA, highlighting their possible health benefits to infants. To assess uptake of milk EVs by intestinal epithelial cells, a method was developed using labelling of isolated EVs with fluorophore-conjugated lactadherin. Lactadherin is a generic and validated EV marker, which enables an effective labelling of phosphatidylserine (PS) exposing EVs. Labelled EVs could effectively be used to describe a dose- and time-dependent uptake into the intestinal epithelial Caco-2 cell line. Additionally, fluorescence microscopy was employed to show that EVs colocalize with endosomal markers and lysosomes, indicating that EVs are taken up via general endocytotic mechanisms. Collectively, a method to specifically label isolated EVs is presented and employed to study the uptake of milk EVs by intestinal epithelial cells.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2020 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2020 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Suiza