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Embryo yield and quality are associated with progestogen treatment during superovulation protocol in lactating Lacaune ewes.
Figueira, Lucas Machado; Alves, Nadja Gomes; Maia, Ana Lucia Rosa E Silva; Souza-Fabjan, Joanna Maria Gonçalves de; Batista, Ribrio Ivan Tavares Pereira; Morais, Maria Clara da Cruz; Lima, Renato Ribeiro de; Oliveira, Maria Emilia Franco; Fonseca, Jeferson Ferreira da.
Afiliación
  • Figueira LM; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil.
  • Alves NG; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil. Electronic address: nadja@ufla.br.
  • Maia ALRES; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil.
  • Souza-Fabjan JMG; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil.
  • Batista RITP; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil.
  • Morais MCDC; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil.
  • Lima RR; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil.
  • Oliveira MEF; Universidade Estadual Paulista "Júlio de Mesquita Filho, Via Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
  • Fonseca JFD; Embrapa Caprinos e Ovinos, Estrada Sobral/ Groaíras, km 4, CP D10, CEP 62011-000, Sobral, CE, Brazil. Electronic address: jeferson.fonseca@embrapa.br.
Theriogenology ; 155: 132-138, 2020 Oct 01.
Article en En | MEDLINE | ID: mdl-32673848
This study assessed the effect of progestogen treatment length on ovarian parameters and embryo yield in superovulated Lacaune ewes collected by nonsurgical embryo recovery. Twenty-three lactating ewes were superovulated 30 d apart using a cross-over design. All ewes received 60 mg of MAP intravaginal sponges for 6 (G-6 group) or 9 (G-9 group) d. A total dose of 133 mg pFSH was given in six decreasing doses (twice a day) starting at 60 h before device removal. Ultrasound examination of the ovaries was performed at the first pFSH injection and one day before embryo recovery, which was performed 6-7 d after the onset of estrus. Embryo recovery was conducted only in ewes that expressed estrus and were mated. There was no difference (P > 0.05) in the total number of follicles between G-6 (15.7 ± 1.0) and G-9 (15.6 ± 0.8) at the time of the first pFSH treatment. The percentage of responding donors with ≥3 corpora lutea (CL; 78.2% [18/23] vs 69.5% [16/23]), mean (±SEM) CL number (7.0 ± 1.2 vs 8.1 ± 1.6), transcervical passage rate (94.4% [17/18] vs 83.3% [15/18], and ova/embryo recovery rate (54.5% [60/110] vs 68.0% [83/122]) were not different (P > 0.05) between the G-6 and G-9 groups. However, the mean number of viable embryos was lower (P < 0.05) in the G-6 group (1.8 ± 0.7) than in the G-9 group. (3.5 ± 1.1). In conclusion, treatment with an intravaginal MAP sponge for 9 d during a superovulation protocol is beneficial for viable embryo yield in Lacaune ewes out of the breeding season.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progestinas / Superovulación Tipo de estudio: Guideline / Risk_factors_studies Límite: Animals Idioma: En Revista: Theriogenology Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progestinas / Superovulación Tipo de estudio: Guideline / Risk_factors_studies Límite: Animals Idioma: En Revista: Theriogenology Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos