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The Effect of ß2-Adrenoceptor Agonists on Leucocyte-Endothelial Adhesion in a Rodent Model of Laparotomy and Endotoxemia.
Bangash, Mansoor Nawaz; Abbott, Tom E F; Patel, Nimesh S A; Hinds, Charles Johnston; Thiemermann, Christoph; Pearse, Rupert Mark.
Afiliación
  • Bangash MN; Department of Critical Care & Anaesthesia, University Hospitals Birmingham NHS Trust, Birmingham, United Kingdom.
  • Abbott TEF; Centre for Translational Medicine & Therapeutics, William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
  • Patel NSA; Centre for Translational Medicine & Therapeutics, William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
  • Hinds CJ; Centre for Translational Medicine & Therapeutics, William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
  • Thiemermann C; Centre for Translational Medicine & Therapeutics, William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
  • Pearse RM; Centre for Translational Medicine & Therapeutics, William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
Front Immunol ; 11: 1001, 2020.
Article en En | MEDLINE | ID: mdl-32670267
Background: The ß2-adrenoceptor agonist dopexamine may possess anti-inflammatory actions which could reduce organ injury during endotoxemia and laparotomy. Related effects on leucocyte-endothelial adhesion remain unclear. Methods: Thirty anesthetized Wistar rats underwent laparotomy followed by induction of endotoxemia with lipopolysaccharide and peptidoglycan (n = 24) or sham (n = 6). Animals received dopexamine at 0.5 or 1 µg kg-1 min-1 (D0.5 and D1), salbutamol at 0.1 µg kg-1 min-1, or saline vehicle (Sham and Control) for 5 h. Intravital microscopy was performed in the ileum of the small intestine to assess leucocyteendothelial adhesion, arteriolar diameter, and functional capillary density. Global hemodynamics and biochemical indices of renal and hepatic function were also measured. Results: Endotoxemia was associated with an increase in adherent leucocytes in post-capillary venules, intestinal arteriolar vasoconstriction as well-reduced arterial pressure and relative cardiac index, but functional capillary density in the muscularis was not significantly altered. Dopexamine and salbutamol administration were associated with reduced leucocyte-endothelial adhesion in post-capillary venules compared to control animals. Arteriolar diameter, arterial pressure and relative cardiac index all remained similar between treated animals and controls. Functional capillary density was similar for all groups. Control group creatinine was significantly increased compared to sham and higher dose dopexamine. Conclusions: In a rodent model of laparotomy and endotoxemia, ß2-agonists were associated with reduced leucocyte-endothelial adhesion in post-capillary venules. This effect may explain some of the anti-inflammatory actions of these agents.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasodilatadores / Dopamina / Endotoxemia / Células Endoteliales / Antagonistas de Receptores Adrenérgicos beta 2 / Leucocitos Límite: Animals / Humans / Male Idioma: En Revista: Front Immunol Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vasodilatadores / Dopamina / Endotoxemia / Células Endoteliales / Antagonistas de Receptores Adrenérgicos beta 2 / Leucocitos Límite: Animals / Humans / Male Idioma: En Revista: Front Immunol Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza