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Impact of Sacbrood Virus on Larval Microbiome of Apis mellifera and Apis cerana.
Yongsawas, Rujipas; Chaimanee, Veeranan; Pettis, Jeffery S; Boncristiani Junior, Humberto Freire; Lopez, Dawn; In-On, Ammarin; Chantawannakul, Panuwan; Disayathanoowat, Terd.
Afiliación
  • Yongsawas R; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Chaimanee V; Department of Agro-Industrial Biotechnology, Maejo University Phrae Campus, Rong Kwang, Phrae 54140, Thailand.
  • Pettis JS; Pettis and Associates LLC, Salisbury, MD 21801, USA.
  • Boncristiani Junior HF; Honey Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, Gainnesville, FL 32611, USA.
  • Lopez D; Bee Research Laboratory, USDA-ARS, Beltsville, MD 20705, USA.
  • In-On A; Bioinformatics & Systems Biology Program, King Mongkut's University of Technology Thonburi (Bang Khun Thian Campus), Bang Khun Thian, Bangkok 10150, Thailand.
  • Chantawannakul P; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Disayathanoowat T; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Insects ; 11(7)2020 Jul 13.
Article en En | MEDLINE | ID: mdl-32668740
In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (Apis mellifera) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, Apis mellifera and Apis cerana. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected A. cerana larvae exhibited a dramatic change compared with A. mellifera. In A. mellifera larvae, the Illumina sequencing revealed the proportion of Gilliamella, Snodgrassella and Fructobacillus was not significantly different, whereas in A. cerana, Gilliamella was significantly decreased (from 35.54% to 2.96%), however, with significant increase in Snodgrassella and Fructobacillus. The possibility of cross-infection should be further investigated.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Insects Año: 2020 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Insects Año: 2020 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza