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Proapoptotic Peptide Brush Polymer Nanoparticles via Photoinitiated Polymerization-Induced Self-Assembly.
Sun, Hao; Cao, Wei; Zang, Nanzhi; Clemons, Tristan D; Scheutz, Georg M; Hu, Ziying; Thompson, Matthew P; Liang, Yifei; Vratsanos, Maria; Zhou, Xuhao; Choi, Wonmin; Sumerlin, Brent S; Stupp, Samuel I; Gianneschi, Nathan C.
Afiliación
  • Sun H; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Cao W; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Zang N; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Clemons TD; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Scheutz GM; Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Chicago, IL, 60611, USA.
  • Hu Z; George & Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science & Engineering, Department of Chemistry, University of Florida, Gainesville, FL, 32611, USA.
  • Thompson MP; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Liang Y; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Vratsanos M; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Zhou X; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Choi W; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Sumerlin BS; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Stupp SI; George & Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science & Engineering, Department of Chemistry, University of Florida, Gainesville, FL, 32611, USA.
  • Gianneschi NC; Department of Chemistry, Department of Materials Science & Engineering, Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Angew Chem Int Ed Engl ; 59(43): 19136-19142, 2020 10 19.
Article en En | MEDLINE | ID: mdl-32659039
Herein, we report the photoinitiated polymerization-induced self-assembly (photo-PISA) of spherical micelles consisting of proapoptotic peptide-polymer amphiphiles. The one-pot synthetic approach yielded micellar nanoparticles at high concentrations and at scale (150 mg mL-1 ) with tunable peptide loadings up to 48 wt. %. The size of the micellar nanoparticles was tuned by varying the lengths of hydrophobic and hydrophilic building blocks. Critically, the peptide-functionalized nanoparticles imbued the proapoptotic "KLA" peptides (amino acid sequence: KLAKLAKKLAKLAK) with two key properties otherwise not inherent to the sequence: 1) proteolytic resistance compared to the oligopeptide alone; 2) significantly enhanced cell uptake by multivalent display of KLA peptide brushes. The result was demonstrated improved apoptosis efficiency in HeLa cells. These results highlight the potential of photo-PISA in the large-scale synthesis of functional, proteolytically resistant peptide-polymer conjugates for intracellular delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Polímeros / Apoptosis / Nanopartículas / Luz Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Polímeros / Apoptosis / Nanopartículas / Luz Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania