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Bacteroides thetaiotaomicron-Infecting Bacteriophage Isolates Inform Sequence-Based Host Range Predictions.
Hryckowian, Andrew J; Merrill, Bryan D; Porter, Nathan T; Van Treuren, William; Nelson, Eric J; Garlena, Rebecca A; Russell, Daniel A; Martens, Eric C; Sonnenburg, Justin L.
Afiliación
  • Hryckowian AJ; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: hryckowian@medicine.wisc.edu.
  • Merrill BD; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Porter NT; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
  • Van Treuren W; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Nelson EJ; Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA.
  • Garlena RA; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Russell DA; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Martens EC; Department of Microbiology & Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
  • Sonnenburg JL; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: jsonnenburg@stanford.edu.
Cell Host Microbe ; 28(3): 371-379.e5, 2020 09 09.
Article en En | MEDLINE | ID: mdl-32652063
Our emerging view of the gut microbiome largely focuses on bacteria, while less is known about other microbial components, such as bacteriophages (phages). Though phages are abundant in the gut, very few phages have been isolated from this ecosystem. Here, we report the genomes of 27 phages from the United States and Bangladesh that infect the prevalent human gut bacterium Bacteroides thetaiotaomicron. These phages are mostly distinct from previously sequenced phages with the exception of two, which are crAss-like phages. We compare these isolates to existing human gut metagenomes, revealing similarities to previously inferred phages and additional unexplored phage diversity. Finally, we use host tropisms of these phages to identify alleles of phage structural genes associated with infectivity. This work provides a detailed view of the gut's "viral dark matter" and a framework for future efforts to further integrate isolation- and sequencing-focused efforts to understand gut-resident phages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Tropismo Viral / Especificidad del Huésped / Bacteroides thetaiotaomicron Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do norte / Asia Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Tropismo Viral / Especificidad del Huésped / Bacteroides thetaiotaomicron Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans País/Región como asunto: America do norte / Asia Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos