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Downregulation of miR-125b-5p and Its Prospective Molecular Mechanism in Lung Squamous Cell Carcinoma.
Huang, Shu-Ping; Jiang, Yi-Fan; Yang, Lin-Jie; Yang, Jie; Liang, Mei-Ting; Zhou, Hua-Fu; Luo, Jiao; Yang, Da-Ping; Mo, Wei-Jia; Chen, Gang; Shi, Lin; Gan, Ting-Qing.
Afiliación
  • Huang SP; Department of Medical Oncology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Jiang YF; Department of Medical Oncology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Yang LJ; Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Yang J; Department of Pharmacology, School of Pharmacy, Guangxi Medical University, Nanning, People's Republic of China.
  • Liang MT; Department of Medical Oncology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Zhou HF; Department of Cardio-Thoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Luo J; Department of Cardio-Thoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Yang DP; Department of Pathology, Guigang People's Hospital of Guangxi/The Eighth Affiliated Hospital of Guangxi Medical University, Guigang, People's Republic of China.
  • Mo WJ; Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Chen G; Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Shi L; Department of Pathology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
  • Gan TQ; Department of Medical Oncology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
Cancer Biother Radiopharm ; 37(2): 125-140, 2022 Mar.
Article en En | MEDLINE | ID: mdl-32614608
Background: To explore the clinical significance of miR-125b-5p and its potential mechanisms in lung squamous cell carcinoma (LUSC). Materials and Methods: An integrated analysis of data from in-house quantitative real-time polymerase chain reaction (qRT-PCR), microRNA-sequencing, and microarray assays to appraise the expression level of miR-125b-5p in LUSC tissues compared to adjacent noncancerous controls. The authors identified the candidate targets of miR-125b-5p and conducted functional analysis using computational biology strategies from gene ontology, the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, disease ontology (DO), and protein-protein interaction (PPI) network analyses to investigate the prospective mechanisms. Results: According to qRT-PCR results, the expression level of miR-125b-5p was markedly decreased in LUSC tissues compared to noncancerous control tissues. Receiver operating characteristic and summary receiver operating characteristic analyses showed that miR-125b-5p had good specificity and sensitivity for distinguishing LUSC tissue from noncancerous lung tissue. The standard mean difference revealed that men and women with lower expression levels of miR-125b-5p may have a higher risk for LUSC. KEGG analysis and DO analysis intimated that target genes were evidently enriched in pyrimidine metabolism and pancreatic carcinoma. The PPI network of the top assembled KEGG pathway indicated that RRM2, UMPS, UCK2, and CTPS1 were regarded as crucial target genes for miR-125b-5p, and RRM2 was eventually deemed a key target. Conclusions: The authors' findings implicate a low expression level of miR-125b-5p in LUSC. A tumor-suppressive role of miR-125b-5p is proposed, based on its effects on LUSC tumor growth, clinical stage progression, and lymph node metastasis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / MicroARNs / Neoplasias Pulmonares Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: Cancer Biother Radiopharm Asunto de la revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / MicroARNs / Neoplasias Pulmonares Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: Cancer Biother Radiopharm Asunto de la revista: FARMACIA / FARMACOLOGIA / NEOPLASIAS / TERAPEUTICA Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos