Purification of Labeled Antibodies Using Size-Exclusion Chromatography.
Cold Spring Harb Protoc
; 2020(7): 099358, 2020 07 01.
Article
en En
| MEDLINE
| ID: mdl-32611782
Many antibody labeling procedures call for a desalting or purification step requiring size-exclusion chromatography (SEC). The method outlined here contains information needed to desalt an antibody conjugate. Similar procedures would be used for ion-exchange chromatography using a gradient of increasing ionic strength. Resins can be purchased in bulk (as in this protocol), or commercially available columns are available.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Cromatografía en Gel
/
Anticuerpos
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Estados Unidos