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Recovery of ovarian function by human embryonic stem cell-derived mesenchymal stem cells in cisplatin-induced premature ovarian failure in mice.
Yoon, Sook Young; Yoon, Jung Ah; Park, Mira; Shin, Eun-Young; Jung, Sookyung; Lee, Jeoung Eun; Eum, Jin Hee; Song, Haengseok; Lee, Dong Ryul; Lee, Woo Sik; Lyu, Sang Woo.
Afiliación
  • Yoon SY; Fertility Center of CHA Gangnam Medical Center, CHA University, 569 Nonhyun-ro, Gangnam-Gu, Seoul, 06125, South Korea.
  • Yoon JA; Fertility Center of CHA Gangnam Medical Center, CHA University, 569 Nonhyun-ro, Gangnam-Gu, Seoul, 06125, South Korea.
  • Park M; Department of Biomedical Science, CHA University, Seongnam-si, South Korea.
  • Shin EY; Department of Biomedical Science, CHA University, Seongnam-si, South Korea.
  • Jung S; CHA Advanced Research Institute, Seongnam-si, South Korea.
  • Lee JE; CHA Advanced Research Institute, Seongnam-si, South Korea.
  • Eum JH; Fertility Center of CHA Gangnam Medical Center, CHA University, 569 Nonhyun-ro, Gangnam-Gu, Seoul, 06125, South Korea.
  • Song H; Department of Biomedical Science, CHA University, Seongnam-si, South Korea.
  • Lee DR; CHA Advanced Research Institute, Seongnam-si, South Korea.
  • Lee WS; Department of Biomedical Science, CHA University, Seongnam-si, South Korea.
  • Lyu SW; Fertility Center of CHA Gangnam Medical Center, CHA University, 569 Nonhyun-ro, Gangnam-Gu, Seoul, 06125, South Korea.
Stem Cell Res Ther ; 11(1): 255, 2020 06 26.
Article en En | MEDLINE | ID: mdl-32586410
BACKGROUND: Clinical use of mesenchymal stem cells (MSCs) requires a uniform cell population, and their harvesting is invasive and produces a limited number of cells. Human embryonic stem cell-derived MSCs (hESC-MSCs) can differentiate into three germ layers and possess immunosuppressive effects in vitro. Anticancer treatment is a well-known risk factor for premature ovarian failure (POF). In this study, we investigated the effect of hESC-MSC on recovery of ovarian function in cisplatin-induced POF in mice. METHODS: Female mice received intraperitoneal cisplatin for 10 days. On day 12, CHA15-derived hESC-MSCs were transplanted into the mice by tail vein injection. An injection of PBS served as the negative control. Ovaries were removed 28 days after transplantation for assessment of ovarian histology, immunostaining, and fertility testing by superovulation and in vitro fertilization. hESC-MSC transplantation into mice with cisplatin-induced damage restored body weight and ovary size. RESULTS: Mean primary and primordial follicle counts in the hESC-MSC group were significantly improved compared to the PBS group (P < 0.05), and counts of zona pellucida remnants, an apoptotic sign in ovarian follicles, were significantly reduced (P < 0.05). TUNEL assays and cleaved PARP immunostaining indicated apoptosis, which led to loss of ovarian stromal cells in negative control mice, while Ki-67 was higher in the hESC-MSC group and in non-cisplatin-treated controls than in the PBS group. Ovulation was reduced in the PBS group but recovered significantly in the hESC-MSC group. Rates of blastocyst formation from ovulated eggs and live births per mouse also recovered significantly in the hESC-MSC group. CONCLUSIONS: hESC-MSC restored structure and function in the cisplatin-damaged ovary. Our study provides new insights into the great clinical potential of human hESC-MSC in treating POF.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insuficiencia Ovárica Primaria / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas / Células Madre Embrionarias Humanas Tipo de estudio: Risk_factors_studies Límite: Animals / Female / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2020 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Insuficiencia Ovárica Primaria / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas / Células Madre Embrionarias Humanas Tipo de estudio: Risk_factors_studies Límite: Animals / Female / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2020 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Reino Unido