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A Hydroxyquinoline-Based Unnatural Amino Acid for the Design of Novel Artificial Metalloenzymes.
Drienovská, Ivana; Scheele, Remkes A; Gutiérrez de Souza, Cora; Roelfes, Gerard.
Afiliación
  • Drienovská I; Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
  • Scheele RA; Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
  • Gutiérrez de Souza C; Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
  • Roelfes G; Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
Chembiochem ; 21(21): 3077-3081, 2020 11 02.
Article en En | MEDLINE | ID: mdl-32585070
We have examined the potential of the noncanonical amino acid (8-hydroxyquinolin-3-yl)alanine (HQAla) for the design of artificial metalloenzymes. HQAla, a versatile chelator of late transition metals, was introduced into the lactococcal multidrug-resistance regulator (LmrR) by stop codon suppression methodology. LmrR_HQAla was shown to complex efficiently with three different metal ions, CuII , ZnII and RhIII to form unique artificial metalloenzymes. The catalytic potential of the CuII -bound LmrR_HQAla enzyme was shown through its ability to catalyse asymmetric Friedel-Craft alkylation and water addition, whereas the ZnII -coupled enzyme was shown to mimic natural Zn hydrolase activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diseño de Fármacos / Metales Pesados / Alanina / Hidroxiquinolinas / Metaloproteínas Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diseño de Fármacos / Metales Pesados / Alanina / Hidroxiquinolinas / Metaloproteínas Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania