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The grapevine NIP2;1 aquaporin is a silicon channel.
Noronha, Henrique; Silva, Angélica; Mitani-Ueno, Namiki; Conde, Carlos; Sabir, Farzana; Prista, Catarina; Soveral, Graça; Isenring, Paul; Ma, Jian Feng; Bélanger, Richard R; Gerós, Hernâni.
Afiliación
  • Noronha H; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Silva A; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.
  • Mitani-Ueno N; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Conde C; Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Japan.
  • Sabir F; i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
  • Prista C; IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
  • Soveral G; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.
  • Isenring P; LEAF, Linking Landscape, Environment, Agriculture and Food, and DRAT, Departamento de Recursos Biológicos, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal.
  • Ma JF; LEAF, Linking Landscape, Environment, Agriculture and Food, and DRAT, Departamento de Recursos Biológicos, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal.
  • Bélanger RR; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal.
  • Gerós H; Nephrology Group, L'Hôtel-Dieu de Québec Institution, Department of Medicine, Faculty of Medicine, Université Laval, Québec, Québec, Canada.
J Exp Bot ; 71(21): 6789-6798, 2020 12 02.
Article en En | MEDLINE | ID: mdl-32584998
Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhão accumulated >0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Freáux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acuaporinas / Vitis Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acuaporinas / Vitis Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido