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Intravital three-dimensional bioprinting.
Urciuolo, Anna; Poli, Ilaria; Brandolino, Luca; Raffa, Paolo; Scattolini, Valentina; Laterza, Cecilia; Giobbe, Giovanni G; Zambaiti, Elisa; Selmin, Giulia; Magnussen, Michael; Brigo, Laura; De Coppi, Paolo; Salmaso, Stefano; Giomo, Monica; Elvassore, Nicola.
Afiliación
  • Urciuolo A; Department of Industrial Engineering, University of Padova, Padova, Italy.
  • Poli I; Veneto Institute of Molecular Medicine, Padova, Italy.
  • Brandolino L; Department of Women's and Children's Health, University of Padova, Padova, Italy.
  • Raffa P; ONYEL Biotech, Padova, Italy.
  • Scattolini V; Department of Industrial Engineering, University of Padova, Padova, Italy.
  • Laterza C; Veneto Institute of Molecular Medicine, Padova, Italy.
  • Giobbe GG; Veneto Institute of Molecular Medicine, Padova, Italy.
  • Zambaiti E; Department of Women's and Children's Health, University of Padova, Padova, Italy.
  • Selmin G; Veneto Institute of Molecular Medicine, Padova, Italy.
  • Magnussen M; Department of Women's and Children's Health, University of Padova, Padova, Italy.
  • Brigo L; Department of Industrial Engineering, University of Padova, Padova, Italy.
  • De Coppi P; Veneto Institute of Molecular Medicine, Padova, Italy.
  • Salmaso S; University College London Great Ormond Street Institute of Child Health, London, UK.
  • Giomo M; Department of Women's and Children's Health, University of Padova, Padova, Italy.
  • Elvassore N; University College London Great Ormond Street Institute of Child Health, London, UK.
Nat Biomed Eng ; 4(9): 901-915, 2020 09.
Article en En | MEDLINE | ID: mdl-32572195
Fabrication of three-dimensional (3D) structures and functional tissues directly in live animals would enable minimally invasive surgical techniques for organ repair or reconstruction. Here, we show that 3D cell-laden photosensitive polymer hydrogels can be bioprinted across and within tissues of live mice, using bio-orthogonal two-photon cycloaddition and crosslinking of the polymers at wavelengths longer than 850 nm. Such intravital 3D bioprinting-which does not create by-products and takes advantage of commonly available multiphoton microscopes for the accurate positioning and orientation of the bioprinted structures into specific anatomical sites-enables the fabrication of complex structures inside tissues of live mice, including the dermis, skeletal muscle and brain. We also show that intravital 3D bioprinting of donor-muscle-derived stem cells under the epimysium of hindlimb muscle in mice leads to the de novo formation of myofibres in the mice. Intravital 3D bioprinting could serve as an in vivo alternative to conventional bioprinting.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Bioimpresión / Impresión Tridimensional Límite: Animals Idioma: En Revista: Nat Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Bioimpresión / Impresión Tridimensional Límite: Animals Idioma: En Revista: Nat Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido