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Soybean recovery from stress imposed by multigenerational growth in contaminated Chernobyl environment.
Pernis, Miroslav; Skultety, Ludovit; Shevchenko, Viktor; Klubicova, Katarina; Rashydov, Namik; Danchenko, Maksym.
Afiliación
  • Pernis M; Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Akademicka 2, 95007 Nitra, Slovakia. Electronic address: miroslav.pernis@savba.sk.
  • Skultety L; Institute of Virology, Biomedical Research Center, Dubravska 9, 84505 Bratislava, Slovakia; Institute of Microbiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague, Czech Republic. Electronic address: viruludo@savba.sk.
  • Shevchenko V; Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Vasylkivska 31/17, 03022 Kyiv, Ukraine. Electronic address: biochemkiev@ukr.net.
  • Klubicova K; Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Akademicka 2, 95007 Nitra, Slovakia. Electronic address: katarina.klubicova@savba.sk.
  • Rashydov N; Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Akademika Zabolotnoho 148, 03143 Kyiv, Ukraine. Electronic address: nrashydov@yahoo.com.
  • Danchenko M; Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Akademicka 2, 95007 Nitra, Slovakia; Institute of Virology, Biomedical Research Center, Dubravska 9, 84505 Bratislava, Slovakia. Electronic address: maksym.danchenko@savba.sk.
J Plant Physiol ; 251: 153219, 2020 Aug.
Article en En | MEDLINE | ID: mdl-32563765
Ionizing radiation is a genotoxic anthropogenic stressor. It can cause heritable changes in the plant genome, which can be either adaptive or detrimental. There is still considerable uncertainty about the effects of chronic low-intensity doses since earlier studies reported somewhat contradictory conclusions. Our project focused on the recovery from the multiyear chronic ionizing radiation stress. Soybean (Glycine max) was grown in field plots located at the Chernobyl exclusion zone and transferred to the clean ground in the subsequent generation. We profiled proteome of mature seeds by two-dimensional gel electrophoresis. Overall, 15 differentially abundant protein spots were identified in the field comparison and 11 in the recovery generation, primarily belonging to storage proteins, disease/defense, and metabolism categories. Data suggested that during multigenerational growth in a contaminated environment, detrimental heritable changes were accumulated. Chlorophyll fluorescence parameters were measured on the late vegetative state, pointing to partial recovery of photosynthesis from stress imposed by contaminating radionuclides. A plausible explanation for the observed phenomena is insufficient provisioning of seeds by lower quality resources, causing a persistent effect in the offspring generation. Additionally, we hypothesized that immunity against phytopathogens was compromised in the contaminated field, but perhaps even primed in the clean ground, yet this idea requires direct functional validation in future experiments. Despite showing clear signs of physiological recovery, one season was not enough to normalize biochemical processes. Overall, our data contribute to the more informed agricultural radioprotection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Radiación Ionizante / Glycine max / Estrés Fisiológico / Proteoma / Accidente Nuclear de Chernóbil País/Región como asunto: Europa Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Radiación Ionizante / Glycine max / Estrés Fisiológico / Proteoma / Accidente Nuclear de Chernóbil País/Región como asunto: Europa Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article Pais de publicación: Alemania