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Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System.
Kamal, Fatima; Liang, Xiaoye; Manera, Kevin; Pei, Tong-Tong; Kim, Haeun; Lam, Linh G; Pun, Alexander; Hersch, Steven J; Dong, Tao G.
Afiliación
  • Kamal F; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Liang X; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jia
  • Manera K; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Pei TT; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Kim H; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Lam LG; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Pun A; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Hersch SJ; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada.
  • Dong TG; Department of Ecosystem and Public Health, University of Calgary, 3330 Hospital Dr. NW, Calgary, AB T2N4Z6, Canada; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jia
Cell Rep ; 31(11): 107766, 2020 06 16.
Article en En | MEDLINE | ID: mdl-32553162
The type VI secretion system (T6SS) is a lethal microbial weapon that injects a large needle-like structure carrying toxic effectors into recipient cells through physical penetration. How recipients respond to physical force and effectors remains elusive. Here, we use a series of effector mutants of Vibrio cholerae to determine how T6SS elicits response in Pseudomonas aeruginosa and Escherichia coli. We show that TseL, but no other effectors or physical puncture, triggers the tit-for-tat response of P. aeruginosa H1-T6SS. Although E. coli is sensitive to all periplasmically expressed effectors, P. aeruginosa is most sensitive to TseL alone. We identify a number of stress response pathways that confer protection against TseL. Physical puncture of T6SS has a moderate inhibitory effect only on envelope-impaired tolB and rseA mutants. Our data reveal that recipient cells primarily respond to effector toxicity but not to physical contact, and they rely on the stress response for immunity-independent protection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Vibrio cholerae / Escherichia coli / Sistemas de Secreción Tipo VI Idioma: En Revista: Cell Rep Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Vibrio cholerae / Escherichia coli / Sistemas de Secreción Tipo VI Idioma: En Revista: Cell Rep Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos