Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis.
Appl Opt
; 59(16): 4849-4855, 2020 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-32543481
Capillary electrophoresis coupled with sheath-flow laser-induced fluorescence (LIF) detection has been shown to offer outstanding sensitivity for chemical and biochemical analysis. However, a major drawback remains with the complexity of the optical configuration traditionally employed. Here we present a simplified confocal optics based on fiber optics and micro gradient-index (GRIN) lenses for modular optical design in capillary electrophoresis with laser-induced fluorescence. We demonstrate the use of the optical system with a sheath-flow cuvette as the laser-induced fluorescence detector for capillary electrophoresis. The system's performance was established with concentration detection limits of 8±2pM and mass detection limits of 57 zeptomole for a standard sodium fluorescein sample.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Appl Opt
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Estados Unidos