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Combined nucleic acid assays for diagnosis of A19 vaccine-caused human brucellosis.
Baoshan, Liu; Yinbo, Ye; Jingbo, Zhai; Yi, Zhang; Jianghua, Yang; Dawei, Cheng; Chi, Ma; Donghai, Yu; Bohan, Yang; Rongnian, Zhu; Sheng, Feng; Jun, Zhang; Han, Xiaohu; Chen, Zeliang.
Afiliación
  • Baoshan L; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Yinbo Y; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Jingbo Z; Innovative Institute of Zoonoses, Inner Mongolia University for the Nationalities, Tongliao, P. R. China.
  • Yi Z; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Jianghua Y; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Dawei C; School of Public Health, Sun Yat-sen University, Guangzhou, P. R. China.
  • Chi M; Brucellosis Department, Heilongjiang Agricultural Reclamation General Hospital, Harbin, P. R. China.
  • Donghai Y; Innovative Institute of Zoonoses, Inner Mongolia University for the Nationalities, Tongliao, P. R. China.
  • Bohan Y; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Rongnian Z; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Sheng F; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Jun Z; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
  • Han X; Brucellosis Department, Heilongjiang Agricultural Reclamation General Hospital, Harbin, P. R. China.
  • Chen Z; Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Shenyang Agricultural University, Shenyang, P. R. China.
Transbound Emerg Dis ; 68(2): 368-374, 2021 Mar.
Article en En | MEDLINE | ID: mdl-32543112
Brucellosis is a common zoonotic disease caused by Brucella and is an epidemic worldwide. Currently, the most effective way to prevent and control the disease in animals is to use live, attenuated vaccines A19 strain. In China, the live attenuated Brucella abortus vaccine is widely used in animal immunization. To detect and confirm which vaccine strain caused the infection, we developed a new method to distinguish A19 strain from non-A19 strains. By comparing the genomic sequences of A19 and wild strain 2,308, we identified signature sequences that are unique to A19. A PCR assay for specific A19 identification was developed based on the genetic marker ABC transporter permease gene. Samples from the outbreak patients were then analysed using the universal quantitative PCR and A19-specific PCR assay, and the A19 strain was successfully identified in them, providing pathogenic evidence of the vaccine-derived infection outbreak. This combined A19-specific differential diagnosis method can provide a means to distinguish between animal vaccine immunization, natural infection and human infection by the vaccine strain. This strategy also has applications in diagnosis, epidemiology and surveillance of A19-related immunizations or infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brucella abortus / Brucelosis / Vacuna contra la Brucelosis / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brucella abortus / Brucelosis / Vacuna contra la Brucelosis / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article Pais de publicación: Alemania