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Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.
Baum, Alina; Fulton, Benjamin O; Wloga, Elzbieta; Copin, Richard; Pascal, Kristen E; Russo, Vincenzo; Giordano, Stephanie; Lanza, Kathryn; Negron, Nicole; Ni, Min; Wei, Yi; Atwal, Gurinder S; Murphy, Andrew J; Stahl, Neil; Yancopoulos, George D; Kyratsous, Christos A.
Afiliación
  • Baum A; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Fulton BO; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Wloga E; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Copin R; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Pascal KE; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Russo V; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Giordano S; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Lanza K; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Negron N; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Ni M; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Wei Y; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Atwal GS; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Murphy AJ; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Stahl N; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Yancopoulos GD; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.
  • Kyratsous CA; Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA. christos.kyratsous@regeneron.com.
Science ; 369(6506): 1014-1018, 2020 08 21.
Article en En | MEDLINE | ID: mdl-32540904
Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Infecciones por Coronavirus / Anticuerpos Neutralizantes / Glicoproteína de la Espiga del Coronavirus / Betacoronavirus / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Science Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Infecciones por Coronavirus / Anticuerpos Neutralizantes / Glicoproteína de la Espiga del Coronavirus / Betacoronavirus / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Science Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos