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Proteome profiling of gestational diabetes mellitus at 16-18 weeks revealed by LC-MS/MS.
Liu, Xiaoting; Sun, Jingru; Wen, Xinyu; Duan, Jinyan; Xue, Dandan; Pan, Yuling; Sun, Jinghua; Zhang, Wei; Cheng, Xiaoliang; Wang, Chengbin.
Afiliación
  • Liu X; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Sun J; Qlife Lab Co., Ltd, Shenzhen, China.
  • Wen X; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Duan J; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Xue D; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Pan Y; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Sun J; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Zhang W; Qlife Lab Co., Ltd, Shenzhen, China.
  • Cheng X; Qlife Lab Co., Ltd, Shenzhen, China.
  • Wang C; Medical School of Chinese PLA & Medical Laboratory Center, First Medical Center of Chinese PLA General Hospital, Beijing, China.
J Clin Lab Anal ; 34(9): e23424, 2020 Sep.
Article en En | MEDLINE | ID: mdl-32537767
BACKGROUND: The practices used to diagnose gestational diabetes mellitus (GDM) could only be carried out around the time of detectable symptoms, and predictive capacity is little. METHODS: LC-MS/MS was conducted to explore overview proteomics for GDM complicated pregnant woman at 16-18 gestation weeks, while normal pregnant for control. Enzyme-linked immunosorbent assay was further applied in an independent cohort of 15 GDM cases and 15 controls for verification. RESULTS: The results indicated that 24 protein expression levels were significantly changed in GDM group samples, and inflammation, oxidative stress, insulin resistance, blood coagulation, and lipid homeostasis were associated with GDM. The abnormal expression of CRP and IGFBP2 was verified in the first-trimester maternal plasma in women who subsequently developed GDM. CONCLUSIONS: This study not only identified 24 potential predictive biomarkers for GDM also provided a global overview of protein rearrangements induced by GDM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Segundo Trimestre del Embarazo / Diabetes Gestacional / Proteoma Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: J Clin Lab Anal Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Segundo Trimestre del Embarazo / Diabetes Gestacional / Proteoma Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: J Clin Lab Anal Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos