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Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response.
Serrano Martinez, Paola; Cinat, Davide; van Luijk, Peter; Baanstra, Mirjam; de Haan, Gerald; Pringle, Sarah; Coppes, Robert P.
Afiliación
  • Serrano Martinez P; Department of Biomedical Sciences of Cells and Systems, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Cinat D; Department of Radiation Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • van Luijk P; Department of Biomedical Sciences of Cells and Systems, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Baanstra M; Department of Radiation Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • de Haan G; Department of Radiation Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Pringle S; Department of Biomedical Sciences of Cells and Systems, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Coppes RP; Department of Radiation Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Oral Dis ; 27(1): 52-63, 2021 Jan.
Article en En | MEDLINE | ID: mdl-32531849
OBJECTIVE: Hyposalivation-related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gland organoid model to explore radiation response of stem cells in vitro. MATERIALS AND METHODS: Single cells derived from murine parotid gland organoids were passaged in Matrigel with defined medium to assess self-renewal and differentiation potential. Single cells were irradiated and plated in a 3D clonogenic stem cell survival assay to assess submandibular and parotid gland radiation response. RESULTS: Single cells derived from parotid gland organoids were able to extensively self-renew and differentiate into all major tissue cell types, indicating the presence of potential stem cells. FACS selection for known salivary gland stem cell markers CD24/CD29 did not further enrich for stem cells. The parotid gland organoid-derived stem cells displayed radiation dose-response curves similar to the submandibular gland. CONCLUSIONS: Murine parotid gland organoids harbor stem cells with long-term expansion and differentiation potential. This model is useful for mechanistic studies of stem cell radiation response and suggests similar radiosensitivity for the parotid and submandibular gland organoids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radiación / Xerostomía / Neoplasias de Cabeza y Cuello Límite: Animals Idioma: En Revista: Oral Dis Asunto de la revista: ODONTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Dinamarca
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radiación / Xerostomía / Neoplasias de Cabeza y Cuello Límite: Animals Idioma: En Revista: Oral Dis Asunto de la revista: ODONTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Dinamarca