Quercetin in the form of a nano-antioxidant (QTiO<sub>2</sub>) provides stabilization of quercetin and maximizes its antioxidant capacity in the mouse fibroblast model.

Birinci, Yelda; Niazi, Javed H; Aktay-Çetin, Oznur; Basaga, Huveyda

Quercetin in the form of a nano-antioxidant (QTiO₂) provides stabilization of quercetin and maximizes its antioxidant capacity in the mouse fibroblast model.

Birinci, Yelda; Niazi, Javed H; Aktay-Çetin, Oznur; Basaga, Huveyda.

Afiliación

Birinci Y; Faculty of Engineering and Natural Sciences, Sabanci University, 34956, Tuzla, Istanbul, Turkey.
Niazi JH; Nanotechnology Research and Application Center (SUNUM), Sabanci University, Tuzla, Istanbul, Turkey.
Aktay-Çetin O; Faculty of Engineering and Natural Sciences, Sabanci University, 34956, Tuzla, Istanbul, Turkey.
Basaga H; Faculty of Engineering and Natural Sciences, Sabanci University, 34956, Tuzla, Istanbul, Turkey. Electronic address: huveyda@sabanciuniv.edu.

Enzyme Microb Technol ; 138: 109559, 2020 Aug.

Article en En | MEDLINE | ID: mdl-32527528

RESUMEN

Living cells are constantly exposed to reactive oxygen species (ROS) causing them to rely on a constant supply of exogenous antioxidants. Quercetin (Q) is one of the potent exogenous antioxidants utilized in various antioxidant formulations. However, the potential application of Q is largely limited because of its poor water solubility. In this study, we employed titanium dioxide (TiO2) nanoparticles to maximize cellular penetration and antioxidant effect of Q on mouse fibroblast cells. To accomplish this, polyethylene glycol (PEG) modified TiO2-nanoparticle surfaces were utilized that exhibited better dispersion, with enhanced biocompatibility. Cell viability assays using Q and Q-conjugated TiO2-nanoparticles (QTiO2) were evaluated in terms of cell morphology as well as with an immunoblotting analysis to look for key biomarkers of apoptosis. In addition, cleavages of Cas 3 and PARP were obtained in cells treated with Q. Furthermore, antioxidant defence with QTiO2 was validated by means of the Nrf2 upregulation pathway. We also observed increased expressions of target enzymes; HO-1, NQO1 and SOD1 in QTiO2-treated cells. The antioxidant potency of the QTiO2 nano-antioxidant form was successfully tested in ROS and superoxide radicals induced cells. Our results demonstrated that the QTiO2 nano-antioxidant promoted a high quercetin bioavailability and stability, in cells with maximal antioxidant potency against ROS, with no signs of cytotoxicity.

Asunto(s)

Antioxidantes/farmacología; Fibroblastos/efectos de los fármacos; Nanopartículas del Metal/química; Quercetina/farmacología; Titanio/química; Animales; Antioxidantes/química; Apoptosis/efectos de los fármacos; Disponibilidad Biológica; Supervivencia Celular/efectos de los fármacos; Fibroblastos/metabolismo; Ratones; Factor 2 Relacionado con NF-E2/genética; Factor 2 Relacionado con NF-E2/metabolismo; Quercetina/química; Especies Reactivas de Oxígeno/metabolismo; Transducción de Señal/efectos de los fármacos; Solubilidad

Palabras clave

Antioxidant therapy; Cleavages of Cas 3 and PARP; Nrf2; Quercetin; ROS; TiO(2)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quercetina / Titanio / Nanopartículas del Metal / Fibroblastos / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Enzyme Microb Technol Año: 2020 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos

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