Your browser doesn't support javascript.
loading
Complex Regulatory Role of the TRPA1 Receptor in Acute and Chronic Airway Inflammation Mouse Models.
Hajna, Zsófia; Cseko, Kata; Kemény, Ágnes; Kereskai, László; Kiss, Tamás; Perkecz, Anikó; Szitter, István; Kocsis, Béla; Pintér, Erika; Helyes, Zsuzsanna.
Afiliación
  • Hajna Z; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Cseko K; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Kemény Á; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Kereskai L; Department of Medical Biology, University of Pécs, Medical School, H-7624 Pécs, Hungary.
  • Kiss T; Department of Pathology, University of Pécs, Medical School, H-7624 Pécs, Hungary.
  • Perkecz A; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Szitter I; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Kocsis B; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
  • Pintér E; Department of Medical Microbiology and Immunology, University of Pécs, Medical School, H-7624 Pécs, Hungary.
  • Helyes Z; Department of Pharmacology and Pharmacotherapy, Medical School and János Szentágothai Research Centre & Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary.
Int J Mol Sci ; 21(11)2020 Jun 09.
Article en En | MEDLINE | ID: mdl-32526913
The Transient Receptor Potential Ankyrin 1 (TRPA1) cation channel expressed on capsaicin-sensitive afferents, immune and endothelial cells is activated by inflammatory mediators and exogenous irritants, e.g., endotoxins, nicotine, crotonaldehyde and acrolein. We investigated its involvement in acute and chronic pulmonary inflammation using Trpa1 gene-deleted (Trpa1-/-) mice. Acute pneumonitis was evoked by intranasal Escherichia coli endotoxin (lipopolysaccharide: LPS) administration, chronic bronchitis by daily cigarette smoke exposure (CSE) for 4 months. Frequency, peak inspiratory/expiratory flows, minute ventilation determined by unrestrained whole-body plethysmography were significantly greater, while tidal volume, inspiratory/expiratory/relaxation times were smaller in Trpa1-/- mice. LPS-induced bronchial hyperreactivity, myeloperoxidase activity, frequency-decrease were significantly greater in Trpa1-/- mice. CSE significantly decreased tidal volume, minute ventilation, peak inspiratory/expiratory flows in wildtypes, but not in Trpa1-/- mice. CSE remarkably increased the mean linear intercept (histopathology), as an emphysema indicator after 2 months in wildtypes, but only after 4 months in Trpa1-/- mice. Semiquantitative histopathological scores were not different between strains in either models. TRPA1 has a complex role in basal airway function regulation and inflammatory mechanisms. It protects against LPS-induced acute pneumonitis and hyperresponsiveness, but is required for CSE-evoked emphysema and respiratory deterioration. Further research is needed to determine TRPA1 as a potential pharmacological target in the lung.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía / Bronquitis Crónica / Canal Catiónico TRPA1 / Fumar Cigarrillos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía / Bronquitis Crónica / Canal Catiónico TRPA1 / Fumar Cigarrillos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Suiza