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Disulphide-mediated site-directed modification of proteins.
Nielsen, Thorbjørn; Märcher, Anders; Drobnáková, Zuzana; Hucko, Michal; Stengl, Milan; Balsánek, Vojtech; Wiberg, Charlotte; Nielsen, Per F; Nielsen, Thomas E; Gothelf, Kurt V; Cló, Emiliano.
Afiliación
  • Nielsen T; Interdisciplinary Nanoscience Center, and the Dept. of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark and Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark. kvg@chem.au.dk.
  • Märcher A; Interdisciplinary Nanoscience Center, and the Dept. of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
  • Drobnáková Z; APIGENEX s.r.o., Podebradská 56/186, Prague 9-180 66, Czech Republic. eclo@novonordisk.com.
  • Hucko M; APIGENEX s.r.o., Podebradská 56/186, Prague 9-180 66, Czech Republic. eclo@novonordisk.com.
  • Stengl M; APIGENEX s.r.o., Podebradská 56/186, Prague 9-180 66, Czech Republic. eclo@novonordisk.com.
  • Balsánek V; APIGENEX s.r.o., Podebradská 56/186, Prague 9-180 66, Czech Republic. eclo@novonordisk.com.
  • Wiberg C; Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark. kvg@chem.au.dk.
  • Nielsen PF; Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark. kvg@chem.au.dk.
  • Nielsen TE; Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark. kvg@chem.au.dk.
  • Gothelf KV; Interdisciplinary Nanoscience Center, and the Dept. of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
  • Cló E; Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark. kvg@chem.au.dk.
Org Biomol Chem ; 18(25): 4717-4722, 2020 07 01.
Article en En | MEDLINE | ID: mdl-32525502
Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hormona del Crecimiento / Disulfuros Límite: Humans Idioma: En Revista: Org Biomol Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hormona del Crecimiento / Disulfuros Límite: Humans Idioma: En Revista: Org Biomol Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido