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Selection based on morphological features of porcine embryos produced by in vitro fertilization: Timing of early cleavages and the effect of polyspermy.
Nguyen, Hiep Thi; Dang-Nguyen, Thanh Quang; Somfai, Tamas; Men, Nguyen Thi; Viet Linh, Nguyen; Xuan Nguyen, Bui; Noguchi, Junko; Kaneko, Hiroyuki; Kikuchi, Kazuhiro.
Afiliación
  • Nguyen HT; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Dang-Nguyen TQ; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
  • Somfai T; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Men NT; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Viet Linh N; Institute of Livestock and Grassland Science, NARO, Tsukuba, Japan.
  • Xuan Nguyen B; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Noguchi J; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Kaneko H; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Kikuchi K; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
Anim Sci J ; 91(1): e13401, 2020.
Article en En | MEDLINE | ID: mdl-32524695
The aim of this study was to examine whether a morphological approach is efficient for selecting high-quality porcine embryos produced by in vitro fertilization (IVF) under high polyspermy conditions. Frozen-thawed Meishan epididymal spermatozoa showing moderate and high polyspermy were subjected to IVF (1 × 105  sperms/ml). Under conditions of moderate polyspermy, 4-cell embryos selected at 48 hr after IVF (single selection) and 8-cell embryos selected at 79 hr after IVF from the collected 4-cell embryos (double selection) showed high developmental competence. Likewise, 4- and 8-cell embryos produced by IVF under high polyspermy conditions also showed high competence for development to blastocysts. However, blastocysts derived from high polyspermy conditions had significantly fewer cells than those produced under moderate polyspermy conditions. Furthermore, the frequency of nuclear and chromosomal abnormalities in 4- and 8-cell embryos produced under conditions of high polyspermy was significantly (p < .05) higher in comparison to moderate polyspermy conditions. These findings suggest that although high polyspermy affects the frequency of nuclear and chromosomal anomalies in porcine IVF embryos, subsequent selection based on morphological features of 4- and 8-cell embryos even under high polyspermy conditions, could be an alternative option for selecting porcine IVF embryos with high development ability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Espermatozoides / Porcinos / Blastocisto / Fertilización In Vitro / Desarrollo Embrionario Límite: Animals Idioma: En Revista: Anim Sci J Asunto de la revista: MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Espermatozoides / Porcinos / Blastocisto / Fertilización In Vitro / Desarrollo Embrionario Límite: Animals Idioma: En Revista: Anim Sci J Asunto de la revista: MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia