Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization.
J Phys Chem Lett
; 11(13): 5212-5218, 2020 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-32515599
We report for the first time that the protonation behavior of weak polyelectrolyte brushes depends very strongly on ionic strength. The pKa changes by one pH step per order of magnitude in salt concentration. For low salt concentrations (â¼1 mM), a very high pH is required to deprotonate a polyacidic brush and a very low pH is required to protonate a polybasic brush. This has major consequences for interactions with other macromolecules, as the brushes are actually almost fully neutral when believed to be charged. We propose that many previous studies on electrostatic interactions between polyelectrolytes and proteins have, in fact, looked at other types of intermolecular forces, in particular, hydrophobic interactions and hydrogen bonds.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Inmovilizadas
/
Polielectrolitos
Idioma:
En
Revista:
J Phys Chem Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Estados Unidos