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Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.
Kuang, Meng-Ting; Li, Jin-Yu; Yang, Xiao-Bei; Yang, Liu; Xu, Jing-Yue; Yan, Sha; Lv, Yong-Feng; Ren, Fu-Cai; Hu, Jiang-Miao; Zhou, Jun.
Afiliación
  • Kuang MT; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
  • Li JY; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
  • Yang XB; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
  • Yang L; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; Bio-Innovation Center of DR PLANT, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan,
  • Xu JY; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China; Bio-Innovation Center of DR
  • Yan S; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; Bio-Innovation Center of DR PLANT, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan,
  • Lv YF; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
  • Ren FC; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
  • Hu JM; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China; Bio-Innovation Center of DR PLANT, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan,
  • Zhou J; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, People's Republic of China. Electronic address: jzhou@mail.kib.ac.cn.
Carbohydr Polym ; 241: 116326, 2020 Aug 01.
Article en En | MEDLINE | ID: mdl-32507202
Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale. Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-ß-d-Glcp-(1→, →4)-ß-d-Manp-(1→, →4)-2-O-acetyl-ß-d-Manp-(1→ and →4)-3-O-acetyl-ß-d-Manp-(1→. In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats. Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor. These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Extractos Vegetales / Dendrobium / Diabetes Mellitus Experimental / Péptido 1 Similar al Glucagón / Hipoglucemiantes Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Extractos Vegetales / Dendrobium / Diabetes Mellitus Experimental / Péptido 1 Similar al Glucagón / Hipoglucemiantes Límite: Animals Idioma: En Revista: Carbohydr Polym Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido