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Electrochemiluminescent sensors as a screening strategy for psychoactive substances within biological matrices.
Brown, Kelly; Jacquet, Charlotte; Biscay, Julien; Allan, Pamela; Dennany, Lynn.
Afiliación
  • Brown K; WESTChem Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. lynn.dennany@strath.ac.uk.
  • Jacquet C; WESTChem Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. lynn.dennany@strath.ac.uk.
  • Biscay J; WESTChem Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. lynn.dennany@strath.ac.uk.
  • Allan P; WESTChem Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. lynn.dennany@strath.ac.uk.
  • Dennany L; WESTChem Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, UK. lynn.dennany@strath.ac.uk.
Analyst ; 145(12): 4295-4304, 2020 Jun 15.
Article en En | MEDLINE | ID: mdl-32500895
With the rapid growth and appearance of novel psychoactive substances (NPS) onto the global drug market, the need for alternative screening methodologies for implementation within clinical environments is substantial. The immunoassay methods currently in use are inadequate for this new drug trend with the potential for misdiagnosis and subsequent administration of incorrect patient treatment increased. This contribution illustrates a strong proof-of-concept for the use of electrochemiluminescence (ECL) as a screening methodology for NPS within biological fluids, using the hallucinogen scopolamine as a model compound. A low cost, easy-to-use and portable sensor has been developed and successfully employed for the detection of scopolamine at clinically relevant concentrations within a variety of biological matrices, including human pooled serum, urine, artificial saliva and sweat, without any prior sample preparation required. Moreover, assessment of the sensor's potential as a point-of-care wearable device was performed with sample collection from the surface of skin, demonstrating its capability for the qualitative identification of scopolamine despite collection of only minimal volumes off the skins surface. The developed sensor described herein exhibits a strong proof-of-concept for the employment of such ECL sensors as point-of-care devices, where the sensors ease of use and removal of time-consuming and complex sample preparation methods will ultimately increase its usability by physicians, widening the avenues where ECL sensors could be employed.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Psicotrópicos / Escopolamina / Sustancias Luminiscentes / Técnicas Electroquímicas / Mediciones Luminiscentes Tipo de estudio: Diagnostic_studies / Qualitative_research / Screening_studies Límite: Humans Idioma: En Revista: Analyst Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Psicotrópicos / Escopolamina / Sustancias Luminiscentes / Técnicas Electroquímicas / Mediciones Luminiscentes Tipo de estudio: Diagnostic_studies / Qualitative_research / Screening_studies Límite: Humans Idioma: En Revista: Analyst Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido