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An Africa-wide genomic evolution of insecticide resistance in the malaria vector Anopheles funestus involves selective sweeps, copy number variations, gene conversion and transposons.
Weedall, Gareth D; Riveron, Jacob M; Hearn, Jack; Irving, Helen; Kamdem, Colince; Fouet, Caroline; White, Bradley J; Wondji, Charles S.
Afiliación
  • Weedall GD; Vector Biology Department, Liverpool School of Tropical Medicine (LSTM), Pembroke Place, Liverpool, United Kingdom.
  • Riveron JM; School of Biological and Environmental Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
  • Hearn J; Vector Biology Department, Liverpool School of Tropical Medicine (LSTM), Pembroke Place, Liverpool, United Kingdom.
  • Irving H; Centre for Research in Infectious Diseases (CRID), Yaoundé, Cameroon.
  • Kamdem C; LSTM Research Unit at CRID, Yaoundé, Cameroon.
  • Fouet C; Vector Biology Department, Liverpool School of Tropical Medicine (LSTM), Pembroke Place, Liverpool, United Kingdom.
  • White BJ; Vector Biology Department, Liverpool School of Tropical Medicine (LSTM), Pembroke Place, Liverpool, United Kingdom.
  • Wondji CS; LSTM Research Unit at CRID, Yaoundé, Cameroon.
PLoS Genet ; 16(6): e1008822, 2020 06.
Article en En | MEDLINE | ID: mdl-32497040
Insecticide resistance in malaria vectors threatens to reverse recent gains in malaria control. Deciphering patterns of gene flow and resistance evolution in malaria vectors is crucial to improving control strategies and preventing malaria resurgence. A genome-wide survey of Anopheles funestus genetic diversity Africa-wide revealed evidences of a major division between southern Africa and elsewhere, associated with different population histories. Three genomic regions exhibited strong signatures of selective sweeps, each spanning major resistance loci (CYP6P9a/b, GSTe2 and CYP9K1). However, a sharp regional contrast was observed between populations correlating with gene flow barriers. Signatures of complex molecular evolution of resistance were detected with evidence of copy number variation, transposon insertion and a gene conversion between CYP6P9a/b paralog genes. Temporal analyses of samples before and after bed net scale up suggest that these genomic changes are driven by this control intervention. Multiple independent selective sweeps at the same locus in different parts of Africa suggests that local evolution of resistance in malaria vectors may be a greater threat than trans-regional spread of resistance haplotypes.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a los Insecticidas / Evolución Molecular / Genoma de los Insectos / Mosquitos Vectores / Malaria / Anopheles Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a los Insecticidas / Evolución Molecular / Genoma de los Insectos / Mosquitos Vectores / Malaria / Anopheles Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos