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TMEM95 is a sperm membrane protein essential for mammalian fertilization.
Lamas-Toranzo, Ismael; Hamze, Julieta G; Bianchi, Enrica; Fernández-Fuertes, Beatriz; Pérez-Cerezales, Serafín; Laguna-Barraza, Ricardo; Fernández-González, Raúl; Lonergan, Pat; Gutiérrez-Adán, Alfonso; Wright, Gavin J; Jiménez-Movilla, María; Bermejo-Álvarez, Pablo.
Afiliación
  • Lamas-Toranzo I; Animal Reproduction Department, INIA, Madrid, Spain.
  • Hamze JG; Department of Cell Biology and Histology, Medical School, University of Murcia, IMIB-Arrixaca, Murcia, Spain.
  • Bianchi E; Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
  • Fernández-Fuertes B; School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
  • Pérez-Cerezales S; Department of Biology, Faculty of Sciences, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
  • Laguna-Barraza R; Animal Reproduction Department, INIA, Madrid, Spain.
  • Fernández-González R; Animal Reproduction Department, INIA, Madrid, Spain.
  • Lonergan P; Animal Reproduction Department, INIA, Madrid, Spain.
  • Gutiérrez-Adán A; School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
  • Wright GJ; Animal Reproduction Department, INIA, Madrid, Spain.
  • Jiménez-Movilla M; Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
  • Bermejo-Álvarez P; Department of Cell Biology and Histology, Medical School, University of Murcia, IMIB-Arrixaca, Murcia, Spain.
Elife ; 92020 06 02.
Article en En | MEDLINE | ID: mdl-32484434
The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for sperm-egg interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this protein were morphologically normal exhibited normal motility, and could penetrate the zona pellucida and bind to the oolemma. However, once bound to the oolemma, TMEM95-deficient sperm were unable to fuse with the egg membrane or penetrate into the ooplasm, and fertilization could only be achieved by mechanical injection of one sperm into the ooplasm, thereby bypassing membrane fusion. These data demonstrate that TMEM95 is essential for mammalian fertilization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Proteínas de Plasma Seminal / Fertilización / Infertilidad Masculina / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Proteínas de Plasma Seminal / Fertilización / Infertilidad Masculina / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido