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Temperature Driven Membrane Lipid Adaptation in Glacial Psychrophilic Bacteria.
Hassan, Noor; Anesio, Alexandre M; Rafiq, Muhammad; Holtvoeth, Jens; Bull, Ian; Haleem, Abdul; Shah, Aamer Ali; Hasan, Fariha.
Afiliación
  • Hassan N; Applied Environmental and Geomicrobiology Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
  • Anesio AM; Bristol Glaciology Centre, School of Geographical Sciences, Faculty of Science, University of Bristol, Bristol, United Kingdom.
  • Rafiq M; Department of Environmental Science, Aarhus University, Roskilde, Denmark.
  • Holtvoeth J; Applied Environmental and Geomicrobiology Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
  • Bull I; Bristol Glaciology Centre, School of Geographical Sciences, Faculty of Science, University of Bristol, Bristol, United Kingdom.
  • Haleem A; Department of Microbiology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan.
  • Shah AA; Organic Geochemistry Unit, School of Chemistry, Cantock's Close, University of Bristol, Bristol, United Kingdom.
  • Hasan F; Organic Geochemistry Unit, School of Chemistry, Cantock's Close, University of Bristol, Bristol, United Kingdom.
Front Microbiol ; 11: 824, 2020.
Article en En | MEDLINE | ID: mdl-32477293
Bacteria inhabiting non-polar glaciers are exposed to large variations in temperature, which significantly affects the fluidity of bacterial cell membranes. In order to maintain normal functions of the cell membranes, psychrophilic bacteria adapt by changing the composition of cell membrane fatty acids. However, information on the exact pattern of cell membrane adaptability in non-polar low-temperature habitats is scarce. In the present study, 42 bacterial strains were isolated from the Ghulmet, Ghulkin, and Hopar glaciers of the Hunza Valley in the Karakoram Mountain Range, Pakistan and their cell membrane fatty acid distributions studied, using gas chromatography/mass spectrometry (GC-MS) for the analysis of fatty acid methyl esters (FAMEs) liberated by acid-catalyzed methanolysis. Furthermore, Gram-negative and Gram-positive groups were grown under different temperature settings (5, 15, 25, and 35°C) in order to determine the effect of temperature on cell membrane (CM) fatty acid distribution. The analyses identified the major groups of cell membrane fatty acids (FA) as straight-chain monounsaturated fatty acids (n-MUFAs) and branched fatty acids (br-FAs), accounting for more than 70% of the fatty acids analyzed. The distribution of br-FAs and n-FAs in bacterial cell membranes was significantly affected by temperature, with the level of br-FAs decreasing relative to n-FAs with increasing temperature. Notably, the production of polyunsaturated fatty acids (PUFAs) was only seen at lower temperatures. This study contributes to understanding, for the first time, the role of br-FAs in the maintenance of cell membrane fluidity of bacteria inhabiting non-polar habitats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza