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Multielemental Analysis of Low-Volume Samples Reveals Cancer-Specific Profile in Serum and Sorted Immune Cells.
Konz, Tobias; Monnard, Caroline; Restrepo, Marcela Rincon; Laval, Julie; Sizzano, Federico; Girotra, Mukul; Dammone, Gabriele; Palini, Alessio; Coukos, George; Rezzi, Serge; Godin, Jean-Philippe; Vannini, Nicola.
Afiliación
  • Konz T; Nestlé Research, Vers-chez-les-Blanc, Lausanne 1000, Switzerland.
  • Monnard C; Nestlé Research, Vers-chez-les-Blanc, Lausanne 1000, Switzerland.
  • Restrepo MR; Laboratory of Immunosenscence and Stem Cell Metabolism, Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, Epalinges 1066, Switzerland.
  • Laval J; Nestlé Research, Vers-chez-les-Blanc, Lausanne 1000, Switzerland.
  • Sizzano F; Nestlé Research, EPFL Innovation Park, Lausanne 1015, Switzerland.
  • Girotra M; Laboratory of Immunosenscence and Stem Cell Metabolism, Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, Epalinges 1066, Switzerland.
  • Dammone G; Nestlé Research, EPFL Innovation Park, Lausanne 1015, Switzerland.
  • Palini A; Nestlé Research, EPFL Innovation Park, Lausanne 1015, Switzerland.
  • Coukos G; Human Integrated Tumor Immunology Discovery Engine, Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, Epalinges 1066, Switzerland.
  • Rezzi S; Nestlé Research, Vers-chez-les-Blanc, Lausanne 1000, Switzerland.
  • Godin JP; Nestlé Research, Vers-chez-les-Blanc, Lausanne 1000, Switzerland.
  • Vannini N; Laboratory of Immunosenscence and Stem Cell Metabolism, Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, Epalinges 1066, Switzerland.
Anal Chem ; 92(13): 8750-8758, 2020 07 07.
Article en En | MEDLINE | ID: mdl-32460479
We developed and validated a reliable, robust, and easy-to-implement quantitative method for multielemental analysis of low-volume samples. Our ICP-MS-based method comprises the analysis of 20 elements (Mg, P, S, K, Ca, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Br, Rb, Sr, Mo, I, Cs, and Ba) in 10 µL of serum and 12 elements (Mg, S, Mn, Fe, Co, Cu, Zn Se, Br, Rb, Mo, and Cs) in less than 250 000 cells. As a proof-of-concept, we analyzed the elemental profiles of serum and sorted immune T cells derived from naïve and tumor-bearing mice. The results indicate a tumor systemic effect on the elemental profiles of both serum and T cells. Our approach highlights promising applications of multielemental analysis in precious samples such as rare cell populations or limited volumes of biofluids that could provide a deeper understanding of the essential role of elements as cofactors in biological and pathological processes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Compuestos Inorgánicos / Neoplasias Límite: Animals Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Compuestos Inorgánicos / Neoplasias Límite: Animals Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos