Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.

Menacho-Melgar, Romel; Ye, Zhixia; Moreb, Eirik A; Yang, Tian; Efromson, John P; Decker, John S; Wang, Ruixin; Lynch, Michael D

Menacho-Melgar, Romel; Ye, Zhixia; Moreb, Eirik A; Yang, Tian; Efromson, John P; Decker, John S; Wang, Ruixin; Lynch, Michael D.

Afiliación

Menacho-Melgar R; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Ye Z; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Moreb EA; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Yang T; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Efromson JP; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Decker JS; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Wang R; Department of Biomedical Engineering, Duke University, Durham, North Carolina.
Lynch MD; Department of Biomedical Engineering, Duke University, Durham, North Carolina.

Biotechnol Bioeng ; 117(9): 2715-2727, 2020 09.

Article en En | MEDLINE | ID: mdl-32441815

Asunto(s)

Reactores Biológicos/microbiología; Escherichia coli; Fosfatos/metabolismo; Ingeniería de Proteínas/métodos; Proteínas Recombinantes/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas Recombinantes/genética

Palabras clave

autoinduction; phosphate depletion; protein expression; stationary phase

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatos / Proteínas Recombinantes / Ingeniería de Proteínas / Reactores Biológicos / Escherichia coli Idioma: En Revista: Biotechnol Bioeng Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

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