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Photografting and Patterning of Poly(ethylene glycol) Methacrylate Hydrogel on Glass for Biochip Applications.
Cepla, Vytautas; Rakickas, Tomas; Stankeviciene, Gintare; Mazetyte-Godiene, Airina; Baradoke, Ausra; Ruzele, Zivile; Valiokas, Ramu Nas.
Afiliación
  • Cepla V; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Rakickas T; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Stankeviciene G; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Mazetyte-Godiene A; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Baradoke A; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Ruzele Z; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
  • Valiokas RN; Department of Nanoengineering, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius, Lithuania.
ACS Appl Mater Interfaces ; 12(29): 32233-32246, 2020 Jul 22.
Article en En | MEDLINE | ID: mdl-32438798
An efficient procedure for chemical initiator-free, in situ synthesis of a functional polyethylene glycol methacrylate (PEG MA) hydrogel on regular glass substrates is reported. It is demonstrated that self-initiated photografting and photopolymerization driven by UV irradiation can yield tens of nanometer-thick coatings of carboxy-functionalized PEG MA on the aldehyde-terminated borosilicate glass surface. The most efficient formulation for hydrogel synthesis contained methyl methacrylic acid (MAA), 2-hydroxyethyl methacrylate (HEMA), and PEG methacrylate (PEG10MA) monomers (1:1:1). The resulting HEMA/PEG10MA/MAA (HPMAA) coatings had a defined thickness in the range from 11 to 50 nm. The physicochemical properties of the synthesized HPMAA coatings were analyzed by combining water contact angle measurements, stylus profilometry, imaging null ellipsometry, and atomic force microscopy (AFM). The latter technique was employed in the quantitative imaging mode not only for direct probing of the surface topography but also for swelling behavior characterization in the pH range from 4.5 to 8.0. The estimated high swelling ratios of the HPMAA hydrogel (up to 3.2) together with its good stability and resistance to nonspecific protein binding were advantageous in extracellular matrix mimetics via patterning of fibronectin (FN) at a resolution close to 200 nm. It was shown that the fabricated FN micropatterns on HPMAA were equally suitable for single-cell arraying, as well as controlled cell culture lasting at least for 96 h.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Técnicas Biosensibles / Materiales Biocompatibles Revestidos / Hidrogeles / Análisis de la Célula Individual / Metacrilatos Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Lituania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Técnicas Biosensibles / Materiales Biocompatibles Revestidos / Hidrogeles / Análisis de la Célula Individual / Metacrilatos Límite: Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Lituania Pais de publicación: Estados Unidos