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Different growth kinetics in blood components and genetic analysis of Lactococcus garvieae isolated from platelet concentrates.
Kozakai, Moe; Matsumoto, Chieko; Matsumoto, Mami; Takakura, Akiko; Matsubayashi, Keiji; Satake, Masahiro.
Afiliación
  • Kozakai M; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
  • Matsumoto C; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
  • Matsumoto M; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
  • Takakura A; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
  • Matsubayashi K; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
  • Satake M; Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Tokyo, Japan.
Transfusion ; 60(7): 1492-1499, 2020 07.
Article en En | MEDLINE | ID: mdl-32436250
BACKGROUND: In 2014, we experienced the first isolation of Lactococcus garvieae from a platelet concentrate (PC). Thereafter, L. garvieae contamination of PCs occurred in two more cases in Japan. It is rare that bacterial contamination with uncommon strains like this species occurs frequently within a short period. Therefore, we performed a detailed analysis of the characteristics of these strains. STUDY DESIGN AND METHODS: Three bacterial strains were identified by biochemical testing and molecular analysis. Genomic diversity was characterized by multilocus sequence typing (MLST). To observe growth kinetics in blood components, PCs were inoculated with the three different strains. RESULTS: All three strains were identified as L. garvieae by molecular analysis. Each strain belonged to a different phylogenetic group according to MLST analysis. In the spiking trial, the three strains demonstrated differences in their final concentrations and changes in appearance of PCs. CONCLUSION: In this study, all three L. garvieae strains were correctly identified by molecular analysis. Since the three strains were collected in different regions of Japan and belonged to different phylogenetic groups according to MLST analysis, it is suggested that L. garvieae have a wide distribution with diversity in Japan. In PCs, the three L. garvieae strains showed clear differences in growth kinetics and changes in appearance of PCs. These differences may have been the primary determinant of whether PC contamination was detected before transfusion. Moreover, L. garvieae represents an emerging foodborne bacterium that can cause transfusion-transmitted bacteremia. Understanding our cases may help prevent bacterial contamination of blood products.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Plaquetas / Lactococcus Límite: Humans Idioma: En Revista: Transfusion Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Plaquetas / Lactococcus Límite: Humans Idioma: En Revista: Transfusion Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos