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Association of mutation and low expression of the CTCF gene with breast cancer progression.
Akhtar, Md Salman; Akhter, Naseem; Najm, Mohammad Zeeshan; Deo, S V S; Shukla, N K; Almalki, Shaia Saleh R; Alharbi, Raed A; Sindi, Abdulmajeed Abdulghani A; Alruwetei, Abdulmohsen; Ahmad, Abrar; Husain, Syed Akhtar.
Afiliación
  • Akhtar MS; Human Genetics Laboratory, Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
  • Akhter N; Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
  • Najm MZ; Human Genetics Laboratory, Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
  • Deo SVS; Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
  • Shukla NK; Department of Biochemistry, Jamia Hamdard, New Delhi 110062, India.
  • Almalki SSR; Department of Surgical Oncology, DR. BRA-IRCH, AIIMS, New Delhi 110029, India.
  • Alharbi RA; Department of Surgical Oncology, DR. BRA-IRCH, AIIMS, New Delhi 110029, India.
  • Sindi AAA; Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
  • Alruwetei A; Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
  • Ahmad A; Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia.
  • Husain SA; Department of Medical Laboratory, College of Applied Medical Sciences, Qassim University, Qassim, Saudi Arabia.
Saudi Pharm J ; 28(5): 607-614, 2020 May.
Article en En | MEDLINE | ID: mdl-32435142
BACKGROUND: CTCF encodes 11-zinc finger protein which is implicated in multiple tumors including the carcinoma of the breast. The Present study investigates the association of CTCF mutations and their expression in breast cancer cases. METHODS: A total of 155 breast cancer and an equal number of adjacent normal tissue samples from 155 breast cancer patients were examined for CTCF mutation(s) by PCR-SSCP and automated DNA sequencing. Immunohistochemistry (IHC) method was used to analyze CTCF expression. Molecular findings were statistically analyzed with various clinicopathological features to identify associations of clinical relevance. RESULTS: Of the total, 16.1% (25/155) cases exhibited mutation in the CTCF gene. Missense mutations Gln > His (G > T) in exon 1 and silent mutations Ser > Ser (C > T) in exon 4 of CTCF gene were analyzed. A significant association was observed between CTCF mutations and some clinicopathological parameters namely menopausal status (p = 0.02) tumor stage (p = 0.03) nodal status (p = 0.03) and ER expression (p = 0.04). Protein expression analysis showed 42.58% samples having low or no expression (+), 38.0% with moderate (++) expression and 19.35% having high (+++) expression for CTCF. A significant association was found between CTCF protein expression and clinicopathological parameters include histological grade (p = 0.04), tumor stage (p = 0.04), nodal status (p = 0.03) and ER status (p = 0.04). CONCLUSIONS: The data suggest that CTCF mutations leading to its inactivation significantly contribute to the progression of breast cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi Pharm J Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Arabia Saudita
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi Pharm J Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Arabia Saudita