NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay.

Nourreddine, Sami; Lavoie, Geneviève; Paradis, Justine; Ben El Kadhi, Khaled; Méant, Antoine; Aubert, Léo; Grondin, Benoit; Gendron, Patrick; Chabot, Benoit; Bouvier, Michel; Carreno, Sébastien; Roux, Philippe P

Nourreddine, Sami; Lavoie, Geneviève; Paradis, Justine; Ben El Kadhi, Khaled; Méant, Antoine; Aubert, Léo; Grondin, Benoit; Gendron, Patrick; Chabot, Benoit; Bouvier, Michel; Carreno, Sébastien; Roux, Philippe P.

Afiliación

Nourreddine S; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Lavoie G; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Paradis J; Moores Cancer Center, University of California, San Diego, La Jolla, CA 92037, USA.
Ben El Kadhi K; Department of Biology, New York University, New York, NY 10003, USA.
Méant A; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Aubert L; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Grondin B; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Gendron P; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada.
Chabot B; Department of Microbiology and Infectious Diseases, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.
Bouvier M; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada; Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Université de Montréal, Montreal, QC H3T 1J4, Canada.
Carreno S; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada; Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montreal, QC H3T 1J4, Canada.
Roux PP; Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, Montreal, QC H3T 1J4, Canada; Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montreal, QC H3T 1J4, Canada. Electronic address: philippe.roux@umontreal.ca.

Cell Rep ; 31(7): 107660, 2020 05 19.

Article en En | MEDLINE | ID: mdl-32433969

RESUMEN

In human cells, the expression of â¼1,000 genes is modulated throughout the cell cycle. Although some of these genes are controlled by specific transcriptional programs, very little is known about their post-transcriptional regulation. Here, we analyze the expression signature associated with all 687 RNA-binding proteins (RBPs) and identify 39 that significantly correlate with cell cycle mRNAs. We find that NF45 and NF90 play essential roles in mitosis, and transcriptome analysis reveals that they are necessary for the expression of a subset of mitotic mRNAs. Using proteomics, we identify protein clusters associated with the NF45-NF90 complex, including components of Staufen-mediated mRNA decay (SMD). We show that depletion of SMD components increases the binding of mitotic mRNAs to the NF45-NF90 complex and rescues cells from mitotic defects. Together, our results indicate that the NF45-NF90 complex plays essential roles in mitosis by competing with the SMD machinery for a common set of mRNAs.

Asunto(s)

Mitosis/fisiología; Proteína del Factor Nuclear 45/metabolismo; Proteínas del Factor Nuclear 90/metabolismo; Estabilidad del ARN/fisiología; Línea Celular Tumoral; Proteínas del Citoesqueleto/genética; Proteínas del Citoesqueleto/metabolismo; Regulación de la Expresión Génica; Células HEK293; Células HeLa; Humanos; Mitosis/genética; Proteínas del Tejido Nervioso/genética; Proteínas del Tejido Nervioso/metabolismo; Proteína del Factor Nuclear 45/genética; Proteínas del Factor Nuclear 90/genética; Estabilidad del ARN/genética; ARN Mensajero/genética; ARN Mensajero/metabolismo; Proteínas de Unión al ARN/genética; Proteínas de Unión al ARN/metabolismo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estabilidad del ARN / Proteínas del Factor Nuclear 90 / Proteína del Factor Nuclear 45 / Mitosis Límite: Humans Idioma: En Revista: Cell Rep Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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