Establishment and Characterization of a Stromal Cell Line Derived From a Patient With Thoracic Endometriosis.

Gogusev, J; Lepelletier, Y; El Khattabi, L; Grigoroiu, M; Validire, P

Gogusev, J; Lepelletier, Y; El Khattabi, L; Grigoroiu, M; Validire, P.

Afiliación

Gogusev J; Cochin Institute, Inserm UMR 1016, CNRS 8104, Université Paris Descartes, 24 rue du faubourg St Jacques, 75014, Paris, France. jean.gogusev@gmail.com.
Lepelletier Y; Imagine Institute, Inserm UMR 1163, CNRS ERL 8254, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
El Khattabi L; Service de Cytogénétique, AP-HP, Hôpital Cochin, Inserm U1016, CNRS 8104, Université Paris Descartes, Paris, France.
Grigoroiu M; Service de Chirurgie Thoracique, Institut Mutualiste Montsouris, Paris, France.
Validire P; Service d'Anatomie Pathologique, Institut Mutualiste Montsouris, Paris, France.

Reprod Sci ; 27(8): 1627-1636, 2020 08.

Article en En | MEDLINE | ID: mdl-32430714

RESUMEN

Thoracic endometriosis (TE) syndrome is a clinical condition known as an extrapelvic form of endometriosis with the presence of functioning endometrial tissue involving lung parenchyma, pleura, chest wall, or diaphragm. In an effort to obtain an endometriosis ex vivo model, we established the spontaneously growing TH-EM1 cell line from endometriotic implants in lung parenchyma from a woman with TE. Maintained in long-term culture, the cells grew as large mesenchymal-like cells with a doubling time between 5 and 6 days. Treatment with medroxyprogesterone acetate (10-7 mol/L) inhibited the TH-EM1 cells growth and induced morphological changes to an epithelial-like cells. Strong expression of the nuclear estrogen receptors, progesterone receptors, and erytropoietin receptors were found in both the pulmonary implant and the TH-EM1 cells by immunohistochemical analysis. Consistent immunoreactivity of TH-EM1 cells for CD9, CD13, CD73, CD90, CD105, and CD157 was revealed by flow cytometry. Likewise, the embryonic markers, SRY-box 2 (SOX-2) and the Nanog molecules, were detected in 76% and 52% of the cells, while fetal hemoglobin and a-globin were detected in 76% and 65% of TH-EM1 cells, respectively. By RHG banding, normal metaphases were observed, while the microarray chromosomal analysis showed gains of DNA sequences located on the segments 8p23.1, 11p15.5, and 12p11.23. The described in vitro cellular model can serve as a useful tool to study the pathogenesis of endometriosis and to improve the knowledge of molecular mechanisms controlling the endometriotic cell dissemination potential.

Asunto(s)

Endometriosis/genética; Endometriosis/patología; Endometrio/patología; Células del Estroma/patología; Enfermedades Torácicas/metabolismo; Enfermedades Torácicas/patología; Adulto; Técnicas de Cultivo de Célula/métodos; Proliferación Celular/fisiología; Diafragma/metabolismo; Diafragma/patología; Endometriosis/metabolismo; Endometrio/metabolismo; Femenino; Humanos; Células del Estroma/metabolismo; Enfermedades Torácicas/genética

Palabras clave

cell line; karyotype; microarray chromosome analysis (MCA); thoracic endometriosis (TE) syndrome

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Torácicas / Células del Estroma / Endometriosis / Endometrio Límite: Adult / Female / Humans Idioma: En Revista: Reprod Sci Asunto de la revista: MEDICINA REPRODUTIVA Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

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