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Pigment epithelium-derived factor (PEDF) and PEDF-receptor in the adult mouse brain: Differential spatial/temporal localization pattern.
de Diego-Otero, Yolanda; Giráldez-Pérez, Rosa María; Lima-Cabello, Elena; Heredia-Farfan, Raúl; Calvo Medina, Rocío; Sanchez-Salido, Lourdes; Pérez Costillas, Lucía.
Afiliación
  • de Diego-Otero Y; Research Laboratory, Hospital Civil, Institute of Biomedical Research in Malaga (IBIMA), Málaga, Spain.
  • Giráldez-Pérez RM; Mental Health Clinic Unit, .Regional University Hospital, Hospital Civil, Málaga, Spain.
  • Lima-Cabello E; Research Unit, International Institute of Innovation and Attention to Neurodevelopment and Language, Málaga, Spain.
  • Heredia-Farfan R; Cellular Biology, Physiology and Immunology Department, University of Cordoba, Edificio Charles Darwin, Córdoba, Spain.
  • Calvo Medina R; Research Laboratory, Hospital Civil, Institute of Biomedical Research in Malaga (IBIMA), Málaga, Spain.
  • Sanchez-Salido L; Research Laboratory, Hospital Civil, Institute of Biomedical Research in Malaga (IBIMA), Málaga, Spain.
  • Pérez Costillas L; Pediatric Clinic Unit. Regional University Hospital, Hospital Materno-Infantil Avd, Arroyo de los Angeles, Málaga, Spain.
J Comp Neurol ; 529(1): 141-158, 2021 01.
Article en En | MEDLINE | ID: mdl-32427349
Pigment epithelium-derived factor (PEDF) is a multifunctional protein which was initially described in the retina, although it is also present in other tissues. It functions as an antioxidant agent promoting neuronal survival. Recently, a PEDF receptor has shown an elevated binding affinity for PEDF. There are no relevant data regarding the distribution of both proteins in the brain, therefore the main goal of this work was to investigate the spatiotemporal presence of PEDF and PEDFR in the adult mouse brain, and to determine the PEDF blood level in mouse and human. The localization of both proteins was analyzed by different experimental methods such as immunohistochemistry, western-blotting, and also by enzyme-linked immunosorbent assay. Differential expression was found in some telencephalic structures and positive signals for both proteins were detected in the cerebellum. The magnitude of the PEDFR labeling pattern was higher than PEDF and included some cortical and subventricular areas. Age-dependent changes in intensity of both protein immunoreactions were found in the cortical and hippocampal areas with greater reactivity between 4 and 8 months of age, whilst others, like the subventricular zones, these differences were more evident for PEDFR. Although ubiquitous presence was not found in the brain for these two proteins, their relevant functions must not be underestimated. It has been described that PEDF plays an important role in neuroprotection and data provided in the present work represents the first extensive study to understand the relevance of these two proteins in specific brain areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Química Encefálica / Serpinas / Receptores de Neuropéptido / Proteínas del Ojo / Factores de Crecimiento Nervioso Límite: Adolescent / Adult / Animals / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: J Comp Neurol Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Química Encefálica / Serpinas / Receptores de Neuropéptido / Proteínas del Ojo / Factores de Crecimiento Nervioso Límite: Adolescent / Adult / Animals / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: J Comp Neurol Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos