Your browser doesn't support javascript.
loading
Seroprevalence and molecular detection of Newcastle disease virus in backyard chickens in Tripoli, Libya.
Gedara, Emhemed A; Alseed, Hesham A; Kammon, Abdulwahab M; Dayhum, Abdunaser S; Benothman, Mohamed A; Al-Garib, Soleman O; Eldaghayes, Ibrahim M.
Afiliación
  • Gedara EA; Department of Microbiology, Faculty of Veterinary Sciences, University of Aljfara, Aljfara, Libya.
  • Alseed HA; Contributed equally in this study.
  • Kammon AM; Department of Microbiology, Higher Institute of Medical Technology, Tripoli, Libya.
  • Dayhum AS; Contributed equally in this study.
  • Benothman MA; Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
  • Al-Garib SO; Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
  • Eldaghayes IM; Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Open Vet J ; 10(1): 80-85, 2020 04.
Article en En | MEDLINE | ID: mdl-32426261
Background: Newcastle disease (ND) is a viral disease that affecting many avian species all over the world. Aim: ND has been successfully controlled by the vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore, this study determined the prevalence of ND in backyard chickens in different locations of Tripoli. Methods: A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time polymerase chain reaction (PCR) and ELISA, respectively. Results: The prevalence of ND virus (NDV) infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using real-time reverse transcription-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter (X2 = 46.13, p ≥ 0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly (X2 = 74.09, p ≥ 0.00001) the highest prevalence (82%) of NDV specific antibodies followed by Tajourah (68%). Conclusion: This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Enfermedad de Newcastle / Pollos / Enfermedad de Newcastle Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Open Vet J Año: 2020 Tipo del documento: Article País de afiliación: Libia Pais de publicación: Libia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Enfermedad de Newcastle / Pollos / Enfermedad de Newcastle Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Open Vet J Año: 2020 Tipo del documento: Article País de afiliación: Libia Pais de publicación: Libia