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Tetracycline resistance transmission in Campylobacter is promoted at temperatures resembling the avian reservoir.
Cuevas-Ferrando, E; Guirado, P; Miró, E; Iglesias-Torrens, Y; Navarro, F; Alioto, T S; Gómez-Garrido, J; Madrid, C; Balsalobre, C.
Afiliación
  • Cuevas-Ferrando E; Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Av. Diagonal, 643, 08028, Barcelona Spain.
  • Guirado P; Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Av. Diagonal, 643, 08028, Barcelona Spain.
  • Miró E; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, Spain.
  • Iglesias-Torrens Y; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, Spain; Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
  • Navarro F; Hospital de la Santa Creu i Sant Pau and Institut d'Investigació Biomèdica Sant Pau (IIB Sant Pau), Barcelona, Spain; Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
  • Alioto TS; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Baldiri i Reixac 4, 08028 Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain.
  • Gómez-Garrido J; CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Baldiri i Reixac 4, 08028 Barcelona, Spain.
  • Madrid C; Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Av. Diagonal, 643, 08028, Barcelona Spain. Electronic address: cmadrid@ub.edu.
  • Balsalobre C; Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Av. Diagonal, 643, 08028, Barcelona Spain. Electronic address: cbalsalobre@ub.edu.
Vet Microbiol ; 244: 108652, 2020 May.
Article en En | MEDLINE | ID: mdl-32402330
Campylobacter is the causal agent of campylobacteriosis in humans, a self-limiting gastroenteritis. Campylobacteriosis is a zoonosis, commonly transmitted from contaminated chicken meat by either direct consumption or cross contamination during food manipulation. Presence of plasmids encoding for resistance to antibiotics such as tetracycline is common among Campylobacter isolates. In this report, we studied the effect of the temperature in the conjugation frequency of several tet(O) carrying plasmids, providing tetracycline resistance to the recipient cells. The conjugation frequency from donor cells carrying three previously characterized plasmids (pCjA13, pCjA9 and pTet) and from two clinical isolates was determined. Two temperatures, 37 and 42 °C, mimicking the conditions encountered by C. jejuni in the human and broiler chicken gastrointestinal tracts, respectively, were assessed. Our results clearly indicate that the conjugation process is promoted at high temperature. Accordingly, the transcriptional expression of some putative conjugative apparatus genes is thermoregulated, being induced at 42 °C. The two plasmids present in the clinical isolates were sequenced and assembled. Both plasmids are highly related among them and to the pTet plasmid. The high identity of the genes putatively involved in the conjugation process among the plasmids is in agreement with the similar behavior regarding the temperature dependency of the conjugative process. This report suggest that conjugation of plasmids carrying antibiotic resistance genes occurs preferentially at temperatures that resemble the gastrointestinal tract of birds, the main reservoir of C. jejuni.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Temperatura Corporal / Campylobacter / Reservorios de Enfermedades / Resistencia a la Tetraciclina Límite: Animals / Humans Idioma: En Revista: Vet Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Temperatura Corporal / Campylobacter / Reservorios de Enfermedades / Resistencia a la Tetraciclina Límite: Animals / Humans Idioma: En Revista: Vet Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos