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Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication.
Santos Neto, Abilio Torres Dos; Silva, Iandara Schettert; Ivo, Maria Lucia; Rodrigues, Camila Tozaki; Parisotto, Eduardo Benedetti; Ramalho, Rondon Tosta; Monteiro, Geanlucas Mendes.
Afiliación
  • Santos Neto ATD; Postgraduate Program in Health and Development in the Midwest Region, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
  • Silva IS; Postgraduate Program in Health and Development in the Midwest Region, Laboratory of Experimental Carcinogenicity, Campo Grande, MS, Brazil.
  • Ivo ML; Postgraduate Program in Health and Development in the Midwest Region, Epidemiological Studies Laboratory, UFMS, Campo Grande, MS, Brazil.
  • Rodrigues CT; School of Nursing, Universidade de São Paulo, Brazil.
  • Parisotto EB; Faculty of Pharmaceutical Sciences, Food and Nutrition, UFMS, Campo Grande, MS, Brazil.
  • Ramalho RT; Postgraduate Program in Health and Development in the Midwest Region, Laboratory of Experimental Carcinogenicity, UFMS, Campo Grande, MS, Brazil.
  • Monteiro GM; Postgraduate Program in Health and Development in the Midwest Region, UFMS, Campo Grande, MS, Brazil.
Acta Cir Bras ; 35(3): e202000301, 2020.
Article en En | MEDLINE | ID: mdl-32401830
PURPOSE: To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. METHODS: Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed. RESULTS: Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. CONCLUSIONS: Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Encéfalo / Estrés Oxidativo / Hidroxiurea / Anemia de Células Falciformes / Hígado Límite: Animals Idioma: En Revista: Acta Cir Bras Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Encéfalo / Estrés Oxidativo / Hidroxiurea / Anemia de Células Falciformes / Hígado Límite: Animals Idioma: En Revista: Acta Cir Bras Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil