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The effect of hematocrit, fibrinogen concentration and temperature on the kinetics of clot formation of whole blood.
Windberger, U; Dibiasi, Ch; Lotz, E M; Scharbert, G; Reinbacher-Koestinger, A; Ivanov, I; Ploszczanski, L; Antonova, N; Lichtenegger, H.
Afiliación
  • Windberger U; Center for Biomedical Research, Medical University Vienna, Vienna, Austria.
  • Dibiasi C; Department of Anaesthesia, Intensive Care Medicine and Pain Medicine, Medical University of Vienna, Vienna, Austria.
  • Lotz EM; Center for Biomedical Research, Medical University Vienna, Vienna, Austria.
  • Scharbert G; Department of Anaesthesia, Intensive Care Medicine and Pain Medicine, Medical University of Vienna, Vienna, Austria.
  • Reinbacher-Koestinger A; Institute of Fundamentals and Theory in Electrical Engineering, Graz University of Technology, Graz, Austria.
  • Ivanov I; Institute of Mechanics, Bulgarian Academy of Science, Sofia, Bulgaria.
  • Ploszczanski L; Department of Material Sciences and Process Engineering, Institute of Physics and Materials Science, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Antonova N; Institute of Mechanics, Bulgarian Academy of Science, Sofia, Bulgaria.
  • Lichtenegger H; Department of Material Sciences and Process Engineering, Institute of Physics and Materials Science, University of Natural Resources and Life Sciences, Vienna, Austria.
Clin Hemorheol Microcirc ; 75(4): 431-445, 2020.
Article en En | MEDLINE | ID: mdl-32390608
BACKGROUND: Dynamic mechanical analysis of blood clots can be used to detect the coagulability of blood. OBJECTIVE: We investigated the kinetics of clot formation by changing several blood components, and we looked into the clot "signature" at its equilibrium state by using viscoelastic and dielectric protocols. METHODS: Oscillating shear rheometry, ROTEM, and a dielectro-rheological device was used. RESULTS: In fibrinogen- spiked samples we found the classical high clotting ability: shortened onset, faster rate of clotting, and higher plateau stiffness. Electron microscopy explained the gain of stiffness. Incorporated RBCs weakened the clots. Reduction of temperature during the clotting process supported the development of high moduli by providing more time for fiber assembly. But at low HCT, clot firmness could be increased by elevating the temperature from 32 to 37°C. In contrast, when the fibrinogen concentration was modified, acceleration of clotting via temperature always reduced clot stiffness, whatever the initial fibrinogen concentration. Electrical resistance increased continuously during clotting; loss tangent (D) (relaxation frequency 249 kHz) decreased when clots became denser: fewer dipoles contributed to the relaxation process. The relaxation peak (Dmax) shifted to lower frequencies at higher platelet count. CONCLUSION: Increasing temperature accelerates clot formation but weakens clots. Rheometry and ROTEM correlate well.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Coagulación Sanguínea / Pruebas de Coagulación Sanguínea / Fibrinógeno / Hematócrito Límite: Adult / Humans / Male Idioma: En Revista: Clin Hemorheol Microcirc Asunto de la revista: ANGIOLOGIA / HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Coagulación Sanguínea / Pruebas de Coagulación Sanguínea / Fibrinógeno / Hematócrito Límite: Adult / Humans / Male Idioma: En Revista: Clin Hemorheol Microcirc Asunto de la revista: ANGIOLOGIA / HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Países Bajos