Your browser doesn't support javascript.
loading
Targeting CD4+ Cells with Anti-CD4 Conjugated Mertansine-Loaded Nanogels.
Canakci, Mine; Singh, Khushboo; Munkhbat, Oyuntuya; Shanthalingam, Sudarvili; Mitra, Ankita; Gordon, Mallory; Osborne, Barbara A; Thayumanavan, S.
Afiliación
  • Canakci M; Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Singh K; Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Munkhbat O; Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Shanthalingam S; Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Mitra A; Center for Bioactive Delivery, Institute for Applied Life Sciences University of Massachusetts, Amherst, Massachusetts 01003, United States.
  • Gordon M; Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Osborne BA; Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
  • Thayumanavan S; Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, Amherst, Massachusetts 10003, United States.
Biomacromolecules ; 21(6): 2473-2481, 2020 06 08.
Article en En | MEDLINE | ID: mdl-32383874
CD4+ T lymphocytes play an important role in controlling many malignancies. The modulation of CD4+ T cells through immunomodulatory or cytotoxic drugs could change the course of disease progression for disorders such as autoimmunity, immunodeficiency, and cancer. Here, we demonstrate that anti-CD4 conjugated polymeric nanogels can deliver a small molecule cargo to primary CD4+ T cells and a CD4high T cell lymphoma. The antibody conjugation not only increased the uptake efficiency of the nanogel (NG) by CD4+ T cells but also decreased the non-specific uptake of the NG by CD4- lymphocytes. For T lymphoma cell lines, the mertansine-loaded conjugate displayed a dose-dependent cell growth inhibition at 17 ng/mL antibody concentration. On the other hand, antibody-drug conjugate (ADC)-type formulation of the anti-CD4 reached similar levels of cell growth inhibition only at the significantly higher concentration of 1.8 µg/mL. NG and antibody conjugates have the advantage of carrying a large payload to a defined target in a more efficient manner as it needs far less antibody to achieve a similar outcome.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoconjugados / Maitansina / Antineoplásicos Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoconjugados / Maitansina / Antineoplásicos Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos