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Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2.
Sahli, Kaouther; Gomri, Mohamed A; Esclapez, Julia; Gómez-Villegas, Patricia; Ghennai, Oulaya; Bonete, María-José; León, Rosa; Kharroub, Karima.
Afiliación
  • Sahli K; Laboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l'Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Constantine, Algeria.
  • Gomri MA; Laboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l'Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Constantine, Algeria.
  • Esclapez J; Agrochemistry and Biochemistry Department, Biochemistry and Molecular Biology Division, Faculty of Science, University of Alicante, Alicante, Spain.
  • Gómez-Villegas P; Laboratory of Biochemistry, Faculty of Experimental Sciences, Marine International Campus of Excellence (CEIMAR), University of Huelva, Huelva, Spain.
  • Ghennai O; Laboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l'Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Constantine, Algeria.
  • Bonete MJ; Agrochemistry and Biochemistry Department, Biochemistry and Molecular Biology Division, Faculty of Science, University of Alicante, Alicante, Spain.
  • León R; Laboratory of Biochemistry, Faculty of Experimental Sciences, Marine International Campus of Excellence (CEIMAR), University of Huelva, Huelva, Spain.
  • Kharroub K; Laboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l'Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Constantine, Algeria.
J Basic Microbiol ; 60(7): 624-638, 2020 Jul.
Article en En | MEDLINE | ID: mdl-32338407
A set of 110 extremely halophilic archaeal strains were isolated from seven distinct saline habitats located in different regions of Algeria. The physicochemical characterization of the samples showed that these habitats were thalassohaline. The carotenoid production from isolated strains varied from 0.1 to 3.68 µg/ml. Based on their physiological characteristics and pigment production, 43 strains were selected and identified by means of phenotypic tests and 16S ribosomal RNA gene sequencing. Phylogenetic analysis indicated that the isolates corresponded to the class Halobacteria and were closely related to genera Halorubrum, Haloarcula, Haloferax, Natrinema, Halogeometricum, Haloterrigena, and Halopiger. Carotenoids of the highest producer, strain Halorubrum sp. BS2 were identified using high-performance liquid chromatography-diode array detector and liquid chromatography-mass spectrometry. Bacterioruberin and bisanhydrobacterioruberin were the predominant carotenoids. The scavenging activity of these carotenoids reached 99% at a concentration of 18 µg/ml, which was much higher than that of ascorbic acid used as a reference compound. These carotenoids also exhibited significant antibacterial activities against four human-pathogenic strains and four fish-pathogenic strains. Variations in salinity, agitation rate, temperature, and light intensity were found to influence growth and carotenoid production of Halorubrum sp. BS2. Our results suggest that halophilic archaea represent a potential source for carotenoids, which are characterized by high antioxidant and antibacterial activities.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Halorubrum / Antibacterianos / Antioxidantes Tipo de estudio: Prognostic_studies País/Región como asunto: Africa Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Halorubrum / Antibacterianos / Antioxidantes Tipo de estudio: Prognostic_studies País/Región como asunto: Africa Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Alemania