Your browser doesn't support javascript.
loading
PD-L1 expression in cell-blocks of non-small cell lung cancer: The impact of prolonged fixation.
Vigliar, Elena; Iaccarino, Antonino; Campione, Severo; Campanino, Maria R; Clery, Eduardo; Pisapia, Pasquale; De Luca, Caterina; Bellevicine, Claudio; Malapelle, Umberto; De Dominicis, Gianfranco; Troncone, Giancarlo.
Afiliación
  • Vigliar E; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Iaccarino A; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Campione S; AORN, Hospital Cardarelli, Naples, Italy.
  • Campanino MR; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Clery E; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Pisapia P; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • De Luca C; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Bellevicine C; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • Malapelle U; Department of Public Health, University of Naples Federico II, Naples, Italy.
  • De Dominicis G; AORN, Hospital Cardarelli, Naples, Italy.
  • Troncone G; Department of Public Health, University of Naples Federico II, Naples, Italy.
Diagn Cytopathol ; 48(7): 595-603, 2020 Jul.
Article en En | MEDLINE | ID: mdl-32333728
INTRODUCTION: In the selection of non-small cell lung cancer (NSCLC) patients for immunotherapy, specimen processed as cell blocks (CBs) may be the only available material to assess PD-L1 expression. Therefore, optimal CB preparation becomes paramount. In this context, here we assessed whether inadequate fixation time might be one of the pre-analytical factors affecting PD-L1 expression. METHODS: Ex vivo CBs from placental (n = 3) and NSCLC (n = 8) resection specimens were obtained. PD-L1 staining was performed on CBs prepared at increasing fixation times (12 hours, 48 hours, 72 hours, 96 hours, 168 hours and 504 hours) using the companion diagnostic SP263 Assay and a validated 22C3 laboratory developed test (LDT). Staining intensity and percentage of positive cells were evaluated. RESULTS: All placental CBs showed moderate to strong PD-L1 positivity in most cells, regardless of the fixation time. Likewise, the percentage of SP263-stained NSCLC cells was similar at all fixation times except for one case, which showed less intense SP263 staining at 168 hours. Conversely, in 5/8 cases, the 22C3 LDT percentage of positive cells and staining intensity decreased at 168 hours and 504 hours. CONCLUSIONS: Our results show that fixation time influences the performance of 22C3 LDT on CBs. Thus, we recommend that the fixation time of cytological materials be carefully checked, especially when PD-L1 testing is delayed until the oncology request. Indeed, delays in tissue processing and paraffin embedding may lead to sub-optimal performance of PD-L1 staining on CBs.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Fijación del Tejido / Carcinoma de Pulmón de Células no Pequeñas / Antígeno B7-H1 / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Diagn Cytopathol Asunto de la revista: PATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Fijación del Tejido / Carcinoma de Pulmón de Células no Pequeñas / Antígeno B7-H1 / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Diagn Cytopathol Asunto de la revista: PATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos