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Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk.
Pecorelli, Ivan; Guarducci, Natascia; von Holst, Cristoph; Bibi, Rita; Pascale, Michelangelo; Ciasca, Biancamaria; Logrieco, Antonio F; Lattanzio, Veronica M T.
Afiliación
  • Pecorelli I; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Salvemini 1, 06126 Perugia, Italy.
  • Guarducci N; Institute of Sciences of Food Production, National Research Council of Italy, via Amendola 122/O, 70126 Bari, Italy.
  • von Holst C; European Commission, Joint Research Centre (JRC), Retieseweg 111, 2440 Geel, Belgium.
  • Bibi R; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Salvemini 1, 06126 Perugia, Italy.
  • Pascale M; Institute of Sciences of Food Production, National Research Council of Italy, via Amendola 122/O, 70126 Bari, Italy.
  • Ciasca B; Institute of Sciences of Food Production, National Research Council of Italy, via Amendola 122/O, 70126 Bari, Italy.
  • Logrieco AF; Institute of Sciences of Food Production, National Research Council of Italy, via Amendola 122/O, 70126 Bari, Italy.
  • Lattanzio VMT; Institute of Sciences of Food Production, National Research Council of Italy, via Amendola 122/O, 70126 Bari, Italy.
Toxins (Basel) ; 12(4)2020 04 22.
Article en En | MEDLINE | ID: mdl-32331441
Aflatoxin B1 (AFB1) is a secondary metabolite produced by some Aspergillus spp. fungi affecting many crops and feed materials. Aflatoxin M1 (AFM1), the 4-hydroxylated metabolite of AFB1, is the main AFB1-related compound present in milk, and it is categorized by the International Agency for Research on Cancer (IARC) as a "group 1 human carcinogen". The aim of this work was to evaluate and compare the analytical performances of two commercial immunoassays widely applied for the detection of AFM1 in milk, namely strip test immunoassay and enzyme linked immunosorbent assay (ELISA). Assay validation included samples at AFM1 levels of 25, 50, 75 ng/kg and blank samples (AFM1 < 0.5 ng/kg). With respect to a screening target concentration (STC) of 50 ng/kg the two assays showed cut-off values of 37.7 ng/kg and 47.5 ng/kg for strip test and ELISA, respectively, a false suspect rate for blanks <0.1% (for both assays) and a false negative rate for samples containing AFM1 at levels higher than STC, of 0.4% (for both assays). The intermediate precision (RSDip) was <32% for the strip test and <15% for the ELISA. Method verification through long-term intra-laboratory quality control (QC) measurements confirmed the results from the validation study. Furthermore, a satisfactory correlation of the results obtained with both immunoassays and the AOAC Official Method 2000.08 was obtained for the analysis of cow milk samples naturally contaminated with AFM1 at levels within "not detected" (< 0.5 ng/kg) and 50 ng/kg. Finally, the extension of the scope of the strip test method to goat and sheep milk was evaluated by applying the experimental design foreseen in the EU regulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Contaminación de Alimentos / Aflatoxina M1 / Leche Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Toxins (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Contaminación de Alimentos / Aflatoxina M1 / Leche Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Toxins (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza