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Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by In Vivo Selection Method.
Ma, Liqiu; Sakamoto, Yoshimitsu; Kanai, Akinori; Otsuka, Hiromi; Takahashi, Akihisa; Kakimi, Kazuhiro; Imai, Takashi; Shimokawa, Takashi.
Afiliación
  • Ma L; National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan.
  • Sakamoto Y; Gunma University Heavy Ion Medical Center, Maebashi, Gunma 371-0034, Japan.
  • Kanai A; China Institute of Atomic Energy, Beijing 102413, China.
  • Otsuka H; National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan.
  • Takahashi A; Graduate School of Science, Toho University, Funabashi, Chiba 274-8510, Japan.
  • Kakimi K; Department of Molecular Oncology and Leukemia Program Project, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 739-8527, Japan.
  • Imai T; National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba 263-8555, Japan.
  • Shimokawa T; Gunma University Heavy Ion Medical Center, Maebashi, Gunma 371-0034, Japan.
Int J Mol Sci ; 21(8)2020 Apr 18.
Article en En | MEDLINE | ID: mdl-32325684
The establishment of cancer cell lines, which have different metastatic abilities compared with the parental cell, is considered as an effective approach to investigate mechanisms of metastasis. A highly metastatic potential mouse colon cancer cell subline, Colon-26MGS, was derived from the parental cell line Colon-26 by in vivo selection using continuous subcutaneous implanting to immunocompetent mice. To clarify the mechanisms involved in the enhancement of metastasis, morphological characteristics, cell proliferation, and gene expression profiles were compared between Colon-26MGS and the parental cell. Colon-26MGS showed over 10 times higher metastatic ability compared with the parental cell, but there were no differences in morphological characteristics and in vitro proliferation rates. In addition, the Colon-26MGS-bearing mice exhibited no marked change of splenocyte population and lung pre-metastatic niche with tumor-free mice, but there were significant differences compared to Colon-26-bearing mice. RNA-seq analyses indicated that immune costimulatory molecules were significantly up-regulated in Colon-26MGS. These results suggest that Colon-26MGS showed not only higher metastatic activity, but also less induction property of host immune response compared to parental Colon-26. Colon-26MGS has proven to be a novel useful tool for studying multiple mechanisms involving metastasis enhancement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma / Movimiento Celular / Neoplasias del Colon / Línea Celular Tumoral / Proliferación Celular / Neoplasias Pulmonares Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma / Movimiento Celular / Neoplasias del Colon / Línea Celular Tumoral / Proliferación Celular / Neoplasias Pulmonares Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza