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A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy.
Vediappan, Rajan Sundaresan; Bennett, Catherine; Bassiouni, Ahmed; Smith, Matthew; Finnie, John; Trochsler, Markus; Psaltis, Alkis J; Vreugde, Sarah; Wormald, Peter J.
Afiliación
  • Vediappan RS; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Bennett C; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Bassiouni A; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Smith M; The Queen Elizabeth Hospital, Animal Experiment Suit, Adelaide, SA, Australia.
  • Finnie J; SA Pathology and Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.
  • Trochsler M; Department of Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Psaltis AJ; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Vreugde S; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
  • Wormald PJ; Department of Surgery - Otolaryngology Head and Neck Surgery, The University of Adelaide, Adelaide, SA, Australia.
Front Surg ; 7: 12, 2020.
Article en En | MEDLINE | ID: mdl-32322586
Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property. Materials and Methods: Twenty-five adult (8-10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology. Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated. Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials / Prognostic_studies Idioma: En Revista: Front Surg Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials / Prognostic_studies Idioma: En Revista: Front Surg Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza